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dc.contributor.authorBurkard, Michael
dc.contributor.authorNash, Susan Bengtson
dc.contributor.authorGambaro, Gessica
dc.contributor.authorWhitworth, Deanne
dc.contributor.authorSchirmer, Kristin
dc.date.accessioned2019-09-30T03:30:52Z
dc.date.available2019-09-30T03:30:52Z
dc.date.issued2019
dc.identifier.issn0742-2091
dc.identifier.doi10.1007/s10565-018-09457-1
dc.identifier.urihttp://hdl.handle.net/10072/387911
dc.description.abstractMarine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular assessments with mammalian wild-type cells are typically constrained by a finite life span, with cells eventually becoming senescent. Thus, the present work explored the possibility of preventing senescence in the HuWa cell line by transfection with plasmids encoding the simian virus large T antigen (SV40T) or telomerase reverse transcriptase (TERT). No stable expression was achieved upon SV40 transfection. Transfection with TERT, on the other hand, activated the expression of telomerase in HuWa cells. At the time of manuscript preparation, the transfected HuWa cells (HuWaTERT) have been stable for at least 59 passages post-transfection. HuWaTERT proliferate rapidly and maintain initial cell characteristics, such as morphology and chromosomal stability. The response of HuWaTERT cells to an immune stimulant (lipopolysaccharide (LPS)) and an immunotoxicant (Aroclor1254) was assessed by measurement of intracellular levels of the pro-inflammatory cytokines interleukin (IL)-6, IL-1β and tumour necrosis factor (TNF)-α. HuWaTERT cells constitutively express IL-6, IL-1β and TNFα. Exposure to neither LPS nor Aroclor1254 had an effect on the levels of these cytokines. Overall, this work supports the diverse applicability of HuWa cell lines in that they display reliable long-term preservation, susceptibility to exogenous gene transfer and enable the study of humpback whale-specific cellular response mechanisms.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofpagefrom387
dc.relation.ispartofpageto398
dc.relation.ispartofissue4
dc.relation.ispartofjournalCell Biology and Toxicology
dc.relation.ispartofvolume35
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3101
dc.subject.keywordsCell line transfection
dc.subject.keywordsHumpback whale
dc.subject.keywordsImmunotoxicity
dc.subject.keywordsInflammatory cytokines
dc.subject.keywordsMegaptera novaeangliae
dc.titleLifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBurkard, M; Bengtson Nash, S; Gambaro, G; Whitworth, D; Schirmer, K, Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor), Cell Biology and Toxicology, 2019, 35 (4), pp. 387-398
dcterms.dateAccepted2018-12-18
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-30T03:23:53Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorBengtson Nash, Susan M.


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