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dc.contributor.authorHan, Pingping
dc.contributor.authorIvanovski, Sašo
dc.date.accessioned2019-10-02T23:03:54Z
dc.date.available2019-10-02T23:03:54Z
dc.date.issued2019
dc.identifier.issn1422-0067
dc.identifier.doi10.3390/ijms20194729
dc.identifier.urihttp://hdl.handle.net/10072/387996
dc.description.abstractDifferent collection methods may influence the ability to detect and quantify biomarker levels in saliva, particularly in the expression of DNA/RNA methylation regulators of several inflammations and tissue turnover markers. This pilot study recruited five participants and unstimulated saliva were collected by either spitting or drooling, and the relative preference for each method was evaluated using a visual analogue scale. Subsequently, total RNA, gDNA and proteins were isolated using the Trizol method. Thereafter, a systematic evaluation was carried out on the potential effects of different saliva collection methods on periodontium-associated genes, DNA/RNA epigenetic factors and periodontium-related DNA methylation levels. The quantity and quality of DNA and RNA were comparable from different collection methods. Periodontium-related genes, DNA/RNA methylation epigenetic factors and periodontium-associated DNA methylation could be detected in the saliva sample, with a similar expression for both methods. The methylation of tumour necrosis factor-alpha gene promoter from drooling method showed a significant positive correlation (TNF α, r = 0.9) with clinical parameter (bleeding on probing-BOP). In conclusion, the method of saliva collection has a minimal impact on detecting periodontium-related genetic and epigenetic regulators in saliva. The pilot data shows that TNF α methylation may be correlated with clinical parameters.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.publisher.placeSwitzerland
dc.relation.ispartofpagefrom4729: 1
dc.relation.ispartofpageto4729: 16
dc.relation.ispartofissue19
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume20
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0699
dc.subject.keywordssaliva collection methods
dc.subject.keywordsDNA methylation
dc.subject.keywordsepigenetic factors
dc.titleEffect of Saliva Collection Methods on the Detection of Periodontium-Related Genetic and Epigenetic Biomarkers-A Pilot Study
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHan, P; Ivanovski, S, Effect of Saliva Collection Methods on the Detection of Periodontium-Related Genetic and Epigenetic Biomarkers-A Pilot Study., International Journal of Molecular Sciences, 2019, 20 (19), pp. 4729: 1-4729: 16
dcterms.dateAccepted2019-09-23
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-10-02T00:33:52Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
gro.hasfulltextFull Text
gro.griffith.authorIvanovski, Saso


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