Intracellular and in Vivo Cyanide Mapping via Surface Plasmon Spectroscopy of Single Au-Ag Nanoboxes
Author(s)
Wang, Peiyuan
Bai, Yujie
Yao, Chi
Li, Xiaomin
Zhou, Lei
Wang, Wenxing
El-Toni, Ahmed Mohamed
Zi, Jian
Zhao, Dongyuan
Shi, Lei
Zhang, Fan
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Cyanide is extremely toxic to organisms but difficult to detect in living biological specimens. Here, we report a new CN - sensing platform based on unmodified Au-Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN - concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference ...
View more >Cyanide is extremely toxic to organisms but difficult to detect in living biological specimens. Here, we report a new CN - sensing platform based on unmodified Au-Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN - concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference time-domain (FDTD) calculations are used to understand the CN - induced spectral shifts.
View less >
View more >Cyanide is extremely toxic to organisms but difficult to detect in living biological specimens. Here, we report a new CN - sensing platform based on unmodified Au-Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN - concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference time-domain (FDTD) calculations are used to understand the CN - induced spectral shifts.
View less >
Journal Title
Analytical Chemistry
Volume
89
Issue
4
Subject
Analytical chemistry
Other chemical sciences
Science & Technology
Physical Sciences
Chemistry, Analytical
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