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dc.contributor.authorSingh, KP
dc.contributor.authorAnwar, S
dc.contributor.authorZaidi, A
dc.contributor.authorSingh, K
dc.contributor.authorDas, P
dc.contributor.authorGourinath, S
dc.contributor.authorAli, V
dc.date.accessioned2019-10-21T00:36:58Z
dc.date.available2019-10-21T00:36:58Z
dc.date.issued2018
dc.identifier.issn0141-8130
dc.identifier.doi10.1016/j.ijbiomac.2018.05.060
dc.identifier.urihttp://hdl.handle.net/10072/388543
dc.description.abstractThe pathogenicity of protozoan parasites is frequently attributed to their ability to circumvent the deleterious effects of ROS and Fe-S clusters are among their susceptible targets with paramount importance for parasite survival. The biogenesis of Fe-S clusters is orchestrated by ISC system; the sulfur donor IscS and scaffold protein IscU being its core components. However, among protozoan parasites including Leishmania, no information is available regarding biochemical aspect of IscU, its interaction partners and regulation. Here, we show that Leishmania donovani IscU homolog, LdIscU, readily assembles [2Fe-2S] clusters and, interestingly, follows Michaelis-Menten enzyme kinetics. It is localized in the mitochondria of the parasite and interacts with LdIscS to form a stable complex. Additionally, LdIscU and Fe-S proteins activity is significantly upregulated in resistant isolates and during stationary growth stage indicating an association between them. The differential expression of LdIscU modulated by Fe-S proteins demand suggests its potential role in parasite survival and drug resistance. Thus, our study provides novel insight into the Fe-S scaffold protein of a protozoan parasite.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom1128
dc.relation.ispartofpageto1145
dc.relation.ispartofjournalInternational Journal of Biological Macromolecules
dc.relation.ispartofvolume116
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode0601
dc.subject.keywordsFe-S scaffold protein
dc.subject.keywordsIron sulfur clusters
dc.subject.keywordsLeishmania
dc.titleLdIscU is a [2Fe-2S] scaffold protein which interacts with LdIscS and its expression is modulated by Fe-S proteins in Leishmania donovani
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSingh, KP; Anwar, S; Zaidi, A; Singh, K; Das, P; Gourinath, S; Ali, V, LdIscU is a [2Fe-2S] scaffold protein which interacts with LdIscS and its expression is modulated by Fe-S proteins in Leishmania donovani, International Journal of Biological Macromolecules, 2018, 116, pp. 1128-1145
dcterms.dateAccepted2018-05-11
dc.date.updated2019-10-21T00:31:46Z
gro.hasfulltextNo Full Text
gro.griffith.authorSingh, Kuljit


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