Size-dependent growth of Microcystis colonies in a shallow, hypertrophic lake: use of the RNA-to-total organic carbon ratio

Abstract

Microcystis was cultured under standard conditions in BG-11 and M-11 media. Using results of an analysis of RNA and total organic carbon (TOC) content, a significant logarithmic relationship between Microcystis growth rate and the RNA/TOC ratio was described to measure the growth rate. Colonial Microcystis samples collected in a shallow, hypertrophic lake (Lake Taihu, China) during May-November 2012 were divided into six size classes (<75, 75-100, 100-150, 150-300, 300-500, and >500 μm), and the RNA/TOC ratio of each class was analyzed to evaluate differences in growth. The growth rate of colonies in the 150-300-μm size class was highest from May to August, but the growth rate increased along with the increase in colony size from September to November. Our results also indicated that water temperature did not change the relationship between growth rate and colony size, but the growth rate of larger colonies was higher than the growth rate of smaller colonies at conditions of low total nitrogen, low total dissolved phosphorus concentration, and high light intensity. Taken together, these results suggest that large colonial Microcystis possess an advantage that is a consequence of this faster growth at lower nutrient concentrations and high light intensities. © 2014 Springer Science+Business Media Dordrecht.