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dc.contributor.authorFraser, TA
dc.contributor.authorBell, MG
dc.contributor.authorHarris, PNA
dc.contributor.authorBell, SC
dc.contributor.authorBergh, H
dc.contributor.authorNguyen, TK
dc.contributor.authorKidd, TJ
dc.contributor.authorNimmo, GR
dc.contributor.authorSarovich, DS
dc.contributor.authorPrice, EP
dc.date.accessioned2019-11-13T04:02:49Z
dc.date.available2019-11-13T04:02:49Z
dc.date.issued2019
dc.identifier.issn2057-5858en_US
dc.identifier.doi10.1099/mgen.0.000307en_US
dc.identifier.urihttp://hdl.handle.net/10072/389128
dc.description.abstractStenotrophomonas maltophilia is emerging as an important cause of disease in nosocomial and community-acquired settings, including bloodstream, wound and catheter-associated infections. Cystic fibrosis (CF) airways also provide optimal growth conditions for various opportunistic pathogens with high antibiotic tolerance, including S. maltophilia. Currently, there is no rapid, cost-effective and accurate molecular method for detecting this potentially life-threatening pathogen, particularly in polymicrobial specimens, suggesting that its true prevalence is underestimated. Here, we used large-scale comparative genomics to identify a specific genetic target for S. maltophilia, with subsequent development and validation of a real-time PCR assay for its detection. Analysis of 167 Stenotrophomonas spp. genomes identified a conserved 4 kb region in S. maltophilia, which was targeted for Black Hole Quencher assay design. Our assay yielded the positive detection of 89 of 89 (100%) clinical S. maltophilia strains, and no amplification of 23 non-S. maltophilia clinical isolates. S. maltophilia was detected in 10 of 16 CF sputa, demonstrating the assay's utility for direct detection in respiratory specimens. The assay demonstrated good sensitivity, with limits of detection and quantitation on pure culture of ~10 and ~100 genome equivalents, respectively. Our assay provides a highly specific, sensitive and cost-effective method for the accurate identification of S. maltophilia, and will improve the diagnosis and treatment of this under-recognized pathogen by enabling its accurate and rapid detection from polymicrobial clinical and environmental samples.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherMicrobiology Societyen_US
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom1en_US
dc.relation.ispartofpageto11en_US
dc.relation.ispartofissue10en_US
dc.relation.ispartofjournalMicrobial genomicsen_US
dc.relation.ispartofvolume5en_US
dc.subject.fieldofresearchMicrobiologyen_US
dc.subject.fieldofresearchcode0605en_US
dc.subject.keywordsStenotrophomonas maltophiliaen_US
dc.subject.keywordscomparative genomicsen_US
dc.subject.keywordsdiagnosticsen_US
dc.titleQuantitative real-time PCR assay for the rapid identification of the intrinsically multidrug-resistant bacterial pathogen Stenotrophomonas maltophiliaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationFraser, TA; Bell, MG; Harris, PNA; Bell, SC; Bergh, H; Nguyen, TK; Kidd, TJ; Nimmo, GR; Sarovich, DS; Price, EP, Quantitative real-time PCR assay for the rapid identification of the intrinsically multidrug-resistant bacterial pathogen Stenotrophomonas maltophilia, Microbial genomics, 2019, 5 (10), pp. 1-11en_US
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2019-11-12T01:22:55Z
dc.description.versionPublisheden_US
gro.rights.copyright© 2019 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
gro.hasfulltextFull Text
gro.griffith.authorNimmo, Graeme R.


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