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dc.contributor.authorHan, Liang
dc.contributor.authorCui, Di
dc.contributor.authorLi, Bin
dc.contributor.authorXu, Wen Wen
dc.contributor.authorLam, Alfred King Y
dc.contributor.authorChan, Kin Tak
dc.contributor.authorZhu, Yun
dc.contributor.authorLee, Nikki PY
dc.contributor.authorLaw, Simon YK
dc.contributor.authorGuan, Xin Yuan
dc.contributor.authorQin, Yan Ru
dc.contributor.authorChan, Kwok Wah
dc.contributor.authorMa, Stephanie
dc.contributor.authorTsao, Sai Wah
dc.contributor.authorCheung, Annie LM
dc.date.accessioned2019-12-02T04:15:48Z
dc.date.available2019-12-02T04:15:48Z
dc.date.issued2019
dc.identifier.issn1347-9032
dc.identifier.doi10.1111/cas.14220
dc.identifier.urihttp://hdl.handle.net/10072/389438
dc.description.abstract5-Fluorouracil (5-FU) is a chemotherapeutic agent commonly used to treat esophageal squamous cell carcinoma (ESCC), but acquisition of chemoresistance frequently occurs and the underlying mechanisms are not fully understood. We found that microRNA (miR)-338-5p was underexpressed in ESCC cells with acquired 5-FU chemoresistance. Forced expression of miR-338-5p in these cells resulted in downregulation of Id-1, and restoration of both in vitro and in vivo sensitivity to 5-FU treatment. The effects were abolished by reexpression of Id-1. In contrast, miR-338-5p knockdown induced 5-FU resistance in chemosensitive esophageal cell lines, and knockdown of both miR-338-5p and Id-1 resensitized the cells to 5-FU. In addition, miR-338-5p had suppressive effects on migration and invasion of ESCC cells. Luciferase reporter assay confirmed a direct interaction between miR-338-5p and the 3'-UTR of Id-1. We also found that miR-338-5p was significantly downregulated in tumor tissue and serum samples of patients with ESCC. Notably, low serum miR-338-5p expression level was associated with poorer survival and poor response to 5-FU/cisplatin-based neoadjuvant chemoradiotherapy. In summary, we found that miR-338-5p can modulate 5-FU chemoresistance and inhibit invasion-related functions in ESCC by negatively regulating Id-1, and that serum miR-338-5p could be a novel noninvasive prognostic and predictive biomarker in ESCC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley Japan
dc.publisher.placeJapan
dc.relation.ispartofjournalCancer Science
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode1112
dc.subject.fieldofresearchcode1115
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordschemoresistance
dc.subject.keywordscirculating miRNA
dc.titleMicroRNA-338-5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id-1
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHan, L; Cui, D; Li, B; Xu, WW; Lam, AKY; Chan, KT; Zhu, Y; Lee, NPY; Law, SYK; Guan, XY; Qin, YR; Chan, KW; Ma, S; Tsao, SW; Cheung, ALM, MicroRNA-338-5p reverses chemoresistance and inhibits invasion of esophageal squamous cell carcinoma cells by targeting Id-1, Cancer Science, 2019
dcterms.dateAccepted2019-10-20
dcterms.licensehttps://creativecommons.org/licenses/by-nc/4.0/
dc.date.updated2019-12-02T03:47:23Z
dc.description.versionVersion of Record (VoR)
gro.description.notepublicThis publication has been entered into Griffith Research Online as an Advanced Online Version
gro.rights.copyright© 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.


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