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dc.contributor.authorAndriamihaja, Mireille
dc.contributor.authorLan, Annaig
dc.contributor.authorBeaumont, Martin
dc.contributor.authorGrauso, Marta
dc.contributor.authorGotteland, Martin
dc.contributor.authorPastene, Edgar
dc.contributor.authorJose Cires, Maria
dc.contributor.authorCarrasco-Pozo, Catalina
dc.contributor.authorTome, Daniel
dc.contributor.authorBlachier, Francois
dc.date.accessioned2019-12-05T01:46:24Z
dc.date.available2019-12-05T01:46:24Z
dc.date.issued2018
dc.identifier.issn0939-4451
dc.identifier.doi10.1007/s00726-018-2558-y
dc.identifier.urihttp://hdl.handle.net/10072/389514
dc.description.abstractHydrogen sulfide (H2S), a metabolic end product synthesized by the microbiota from l-cysteine, has been shown to act at low micromolar concentration as a mineral oxidative substrate in colonocytes while acting as an inhibitor of oxygen consumption at higher luminal concentrations (65 µM and above). From the previous works showing that polyphenols can bind volatile sulfur compounds, we hypothesized that different dietary proanthocyanidin-containing polyphenol (PACs) plant extracts might modulate the inhibitory effect of H2S on colonocyte respiration. Using the model of human HT-29 Glc–/+ cell colonocytes, we show here that pre-incubation of 65 µM of the H2S donor NaHS with the different polyphenol extracts markedly reduced the inhibitory effect of NaHS on colonocyte oxygen consumption. Our studies on HT-29 Glc–/+ cell respiration performed in the absence or the presence of PACs reveal rapid binding of H2S with the sulfide-oxidizing unit and slower binding of H2S to the cytochrome c oxidase (complex IV of the respiratory chain). Despite acute inhibition of colonocyte respiration, no measurable effect of NaHS on paracellular permeability was recorded after 24 h treatment using the Caco-2 colonocyte monolayer model. The results are discussed in the context of the binding of excessive bacterial metabolites by unabsorbed dietary compounds and of the capacity of colonocytes to adapt to changing luminal environment.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofpagefrom755
dc.relation.ispartofpageto763
dc.relation.ispartofissue6
dc.relation.ispartofjournalAmino Acids
dc.relation.ispartofvolume50
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode1101
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsHydrogen sulfide
dc.subject.keywordsProanthocyanidins
dc.titleProanthocyanidin-containing polyphenol extracts from fruits prevent the inhibitory effect of hydrogen sulfide on human colonocyte oxygen consumption
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAndriamihaja, M; Lan, A; Beaumont, M; Grauso, M; Gotteland, M; Pastene, E; Jose Cires, M; Carrasco-Pozo, C; Tome, D; Blachier, F, Proanthocyanidin-containing polyphenol extracts from fruits prevent the inhibitory effect of hydrogen sulfide on human colonocyte oxygen consumption, Amino Acids, 2018, 50 (6), pp. 755-763
dcterms.dateAccepted2018-03-24
dc.date.updated2019-12-05T00:35:39Z
gro.hasfulltextNo Full Text
gro.griffith.authorCarrasco Pozo, Catalina A.


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