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  • Differential protective effects of quercetin, resveratrol, rutin and epigallocatechin gallate against mitochondrial dysfunction induced by indomethacin in Caco-2 cells

    Author(s)
    Carrasco-Pozo, Catalina
    Luisa Mizgier, Maria
    Speisky, Hernan
    Gotteland, Martin
    Griffith University Author(s)
    Carrasco Pozo, Catalina A.
    Year published
    2012
    Metadata
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    Abstract
    The beneficial effects of dietary polyphenols on health are due not only to their antioxidant properties but also to their antibacterial, anti-inflammatory and/or anti-tumoral activities. It has recently been proposed that protection of mitochondrial function (which is altered in several diseases such as Alzheimer, Parkinson, obesity and diabetes) by these compounds, may be important in explaining the beneficial effects of polyphenols on health. The aim of this study was to evaluate the protective effects of dietary polyphenols quercetin, rutin, resveratrol and epigallocatechin gallate against the alterations of mitochondrial ...
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    The beneficial effects of dietary polyphenols on health are due not only to their antioxidant properties but also to their antibacterial, anti-inflammatory and/or anti-tumoral activities. It has recently been proposed that protection of mitochondrial function (which is altered in several diseases such as Alzheimer, Parkinson, obesity and diabetes) by these compounds, may be important in explaining the beneficial effects of polyphenols on health. The aim of this study was to evaluate the protective effects of dietary polyphenols quercetin, rutin, resveratrol and epigallocatechin gallate against the alterations of mitochondrial function induced by indomethacin (INDO) in intestinal epithelial Caco-2 cells, and to address the mechanism involved in such damaging effect by INDO, which generates oxidative stress. INDO concentration dependently decreases cellular ATP levels and mitochondrial membrane potential in Caco-2 cells after 20 min of incubation. INDO also inhibits the activity of mitochondrial complex I and causes accumulation of NADH; leading to overproduction of mitochondrial O 2•-, since it is prevented by pyruvate. Quercetin (0.01 mg/ml), resveratrol (0.1 mg/ml) and rutin (1 mg/ml) protected Caco-2 cells against INDO-induced mitochondrial dysfunction, while no protection was observed with epigallocatechin gallate. Quercetin was the most efficient in protecting against mitochondrial dysfunction; this could be due to its ability to enter cells and accumulate in mitochondria. Additionally its structural similarity with rotenone could favor its binding to the ubiquinone site of complex I, protecting it from inhibitors such as INDO or rotenone. These findings suggest a possible new protective role for dietary polyphenols for mitochondria, complementary of their antioxidant property. This new role might expand the preventive and/or therapeutic use of PPs in conditions involving mitochondrial dysfunction and associated with increased oxidative stress at the cellular or tissue levels. © 2011 Elsevier Ireland Ltd. All rights reserved.
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    Journal Title
    Chemico-Biological Interactions
    Volume
    195
    Issue
    3
    DOI
    https://doi.org/10.1016/j.cbi.2011.12.007
    Subject
    Biochemistry and Cell Biology
    Science & Technology
    Life Sciences & Biomedicine
    Biochemistry & Molecular Biology
    Pharmacology & Pharmacy
    Toxicology
    Publication URI
    http://hdl.handle.net/10072/389528
    Collection
    • Journal articles

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