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dc.contributor.authorLopez-Alarcon, C
dc.contributor.authorRocco, C
dc.contributor.authorLissi, E
dc.contributor.authorCarrasco, C
dc.contributor.authorSquella, JA
dc.contributor.authorNunez-Vergara, L
dc.contributor.authorSpeisky, H
dc.description.abstractPURPOSE: The aims of the study are to analyze the interaction between 5-aminosalicylic acid (5-ASA) and peroxyl radicals and to evaluate the effect of some endogenous compounds such as ascorbic acid and amino acids on the oxidation of 5-ASA induced by 2,2'-azo-bis(2-amidinopropane) dihydrochloride. METHODS: The consumption and/or the recovery of 5-ASA (7.6 microM) exposed to a peroxyl radical source [2,2'-azo-bis(2-amidinopropane)] was followed by techniques such as spectrofluorescence, high-performance liquid chromatography, and differential pulse voltammetry. RESULTS: 5-Aminosalicylic acid was found to readily react with peroxyl radicals at micromolar concentrations and to protect c-Phycocyanin in a very similar fashion to that shown by Trolox. Exposure of 5-ASA to peroxyl radicals led to its oxidation into the corresponding quinone-imine. Disappearance of 5-ASA was prevented by tryptophan, cysteine, glutathione, and ascorbic acid. Furthermore, some of these compounds induced the partial (cysteine and glutathione) or total (ascorbic acid) recovery of 5-ASA when added after its almost total consumption. CONCLUSIONS: 5-Aminosalicylic acid is a very efficient peroxyl radical scavenger. The 5-ASA oxidation by peroxyl radicals was prevented by ascorbic acid, cysteine, and glutathione. In addition, 5-ASA can be regenerated by these endogenous compounds, which would be a valuable mechanism to preserve 5-ASA in tissues undergoing oxidative stress conditions.
dc.relation.ispartofjournalPharmaceutical Research
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsChemistry, Multidisciplinary
dc.subject.keywordsPharmacology & Pharmacy
dc.titleReaction of 5-aminosalicylic acid with peroxyl radicals: Protection and recovery by ascorbic acid and amino acids
dc.typeJournal article
dcterms.bibliographicCitationLopez-Alarcon, C; Rocco, C; Lissi, E; Carrasco, C; Squella, JA; Nunez-Vergara, L; Speisky, H, Reaction of 5-aminosalicylic acid with peroxyl radicals: Protection and recovery by ascorbic acid and amino acids, Pharmaceutical Research, 2005, 22 (10), pp. 1642-1648
gro.hasfulltextNo Full Text
gro.griffith.authorCarrasco Pozo, Catalina A.

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