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dc.contributor.authorSreevatsav Adiraju, SK
dc.contributor.authorShekar, K
dc.contributor.authorFraser, JF
dc.contributor.authorSmith, MT
dc.contributor.authorGhassabian, S
dc.date.accessioned2019-12-12T03:34:11Z
dc.date.available2019-12-12T03:34:11Z
dc.date.issued2018
dc.identifier.issn1757-6180
dc.identifier.doi10.4155/bio-2018-0102
dc.identifier.urihttp://hdl.handle.net/10072/389719
dc.description.abstractAim: To develop an LC-MS/MS assay to quantitate well-tolerated substrates; midazolam (CYP3A), omeprazole (CYP2C19), dextromethorphan (CYP2D6), losartan (CYP2C9) and their respective metabolites' concentrations in plasma samples. Patients & methods: A solid-phase extraction method was optimized to extract analytes of interest simultaneously from human plasma samples. The assay analyzed plasma samples collected from patients who received equal or lower than therapeutic doses of CYP substrates. Results: This assay was validated based on the European Medicines Agency guideline for bioanalytical method validation and was sensitive, linear, accurate and precise with acceptable recovery and matrix effects. Conclusion: Small sample volume and dose of cytochrome P450 substrates, short-run time, using stable isotope internal standards and being cost effective are the major advantages of the assay.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherFuture Science Ltd
dc.relation.ispartofpagefrom1577
dc.relation.ispartofpageto1590
dc.relation.ispartofissue19
dc.relation.ispartofjournalBioanalysis
dc.relation.ispartofvolume10
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode1115
dc.subject.keywordsCYP phenotyping
dc.subject.keywordsCYP2C19
dc.subject.keywordsCYP2C9
dc.subject.keywordsCYP2D6
dc.subject.keywordsCYP3A4
dc.titleAn improved LC-MS/MS method for simultaneous evaluation of CYP2C9, CYP2C19, CYP2D6 and CYP3A4 activity
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSreevatsav Adiraju, SK; Shekar, K; Fraser, JF; Smith, MT; Ghassabian, S, An improved LC-MS/MS method for simultaneous evaluation of CYP2C9, CYP2C19, CYP2D6 and CYP3A4 activity, Bioanalysis, 2018, 10 (19), pp. 1577-1590
dc.date.updated2019-12-12T03:32:53Z
gro.hasfulltextNo Full Text
gro.griffith.authorFraser, John F.


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