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dc.contributor.convenorGillian Buckley
dc.contributor.authorLaakso, Liisa
dc.contributor.authorPowell, Katie
dc.contributor.authorMcDonnell, Ann
dc.contributor.authorRalph, Stephen
dc.contributor.editorL. Laakso
dc.description.abstractBackground: Despite limited safety data, Low Level Laser Therapy (LLLT) is used in the clinical treatment of breast cancer-related lymphoedema (BCRL). Objective: We aimed to compare the cell proliferative effects of a range of doses of LLLT at wavelengths of 780, 830 and 904 nm on human breast and immortalised human mammary epithelial cell lines in vitro. Materials and Methods: Human breast adenocarcinoma (MCF-7), human melanoma cell line (MDA-MB-435S/M14) and immortalised human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of LASER at 0.5, 1, 2, 3, 4, 10 and 12 J/cm2 (?=780 nm) and 0.5, 1, 2, 3, 4, 10 and 15 J/cm2 (?=830 and 904 nm). MCF-7 cells were further irradiated with two and three exposures of all wavelengths. Cell proliferation was assessed 24 hours after irradiation. Results: SVCT cell proliferation significantly increased after exposure to a range of doses at 780 and 904 nm irradiation. MDA-MB-435S and Bre80hTERT cell lines showed negligible effects to LASER exposure. MCF-7 cells irradiated with 780 nm LASER demonstrated an increasing dose response relationship after one exposure and a decreasing dose response relationship after three exposures. MCF-7 cells irradiated with 904 nm LASER demonstrated a decreasing dose response relationship after two and three exposures. Conclusion: Although certain LASER doses increased MCF-7 cell proliferation, multiple exposures had no effect or a decreasing effect on dose response relationships. Before a definitive conclusion can be made regarding LASER safety for BCRL, further in vivo research is necessary.
dc.publisherAustralasian Lymphology Association
dc.relation.ispartofconferencename8th Australasian Lymphology Association Conference
dc.relation.ispartofconferencetitle8th Australasian Lymphology Association Conference: Reflections on the past, Inspirations for the future
dc.relation.ispartoflocationMelbourne, Australia
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.titleThe in vitro effects of LASER on human mammary epithelial and human breast cancer cell lines
dc.typeConference output
dc.type.descriptionE1 - Conferences
dc.type.codeE - Conference Publications
gro.facultyGriffith Health, School of Allied Health Sciences
gro.hasfulltextNo Full Text
gro.griffith.authorMcDonnell, Ann A.
gro.griffith.authorLaakso, Liisa
gro.griffith.authorRalph, Stephen J.
gro.griffith.authorLow, Pauline
gro.griffith.authorPowell, Katie

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    Contains papers delivered by Griffith authors at national and international conferences.

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