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dc.contributor.authorWang, T
dc.contributor.authorMa, G
dc.contributor.authorAng, CS
dc.contributor.authorKorhonen, PK
dc.contributor.authorStroehlein, AJ
dc.contributor.authorYoung, ND
dc.contributor.authorHofmann, A
dc.contributor.authorChang, BCH
dc.contributor.authorWilliamson, NA
dc.contributor.authorGasser, RB
dc.date.accessioned2020-01-07T03:33:12Z
dc.date.available2020-01-07T03:33:12Z
dc.date.issued2020
dc.identifier.issn1874-3919
dc.identifier.doi10.1016/j.jprot.2019.103615
dc.identifier.urihttp://hdl.handle.net/10072/390076
dc.description.abstractProtein phosphorylation plays essential roles in many cellular processes. Despite recent progress in the genomics, transcriptomics and proteomics of socioeconomically important parasitic nematodes, there is scant phosphoproteomic data to underpin molecular biological discovery. Here, using the phosphopeptide enrichment-based LC-MS/MS and data-independent acquisition (DIA) quantitation, we characterised the first developmental phosphoproteome of the parasitic nematode Haemonchus contortus – one of the most pathogenic parasites of ruminant livestock. Totally, 1804 phosphorylated proteins with 4406 phosphorylation sites (‘phosphosites’) from different developmental stages/sexes were identified. Bioinformatic analyses of quantified ‘phosphosites’ exhibited distinctive stage- and sex-specific patterns during development, and identified a subset of phosphoproteins proposed to play crucial roles in processes such as spindle positioning, signal transduction and kinase activity. A sequence-based comparison of the phosphoproteome of H. contortus with those of two free-living nematode species (Caenorhabditis elegans and Pristionchus pacificus) suggested a limited number of common protein phosphorylation events among these species. Our findings infer active roles for protein phosphorylation in the adaptation of a parasitic nematode to a constantly changing external environment. The phosphoproteomic data set for H. contortus provides a basis to better understand phosphorylation and associated biological processes (e.g., regulation of signal transduction), and might enable the discovery of novel anthelmintic targets.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofpagefrom103615: 1
dc.relation.ispartofpageto103615: 10
dc.relation.ispartofjournalJournal of Proteomics
dc.relation.ispartofvolume213
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3108
dc.titleThe developmental phosphoproteome of Haemonchus contortus
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationWang, T; Ma, G; Ang, CS; Korhonen, PK; Stroehlein, AJ; Young, ND; Hofmann, A; Chang, BCH; Williamson, NA; Gasser, RB, The developmental phosphoproteome of Haemonchus contortus, Journal of Proteomics, 2020, 213, pp. 103615: 1-103615: 10
dc.date.updated2020-01-06T23:54:31Z
gro.hasfulltextNo Full Text
gro.griffith.authorHofmann, Andreas


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