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dc.contributor.authorAbbasi, Naghmeh
dc.contributor.authorIvanovski, Saso
dc.contributor.authorGulati, Karan
dc.contributor.authorLove, Robert M
dc.contributor.authorHamlet, Stephen
dc.date.accessioned2020-01-10T05:00:21Z
dc.date.available2020-01-10T05:00:21Z
dc.date.issued2020
dc.identifier.issn2055-7124
dc.identifier.doi10.1186/s40824-019-0180-z
dc.identifier.urihttp://hdl.handle.net/10072/390111
dc.description.abstractBackground: Cell-scaffold based therapies have the potential to offer an efficient osseous regenerative treatment and PCL has been commonly used as a scaffold, however its effectiveness is limited by poor cellular retention properties. This may be improved through a porous scaffold structure with efficient pore arrangement to increase cell entrapment. To facilitate this, melt electrowriting (MEW) has been developed as a technique able to fabricate cell-supporting scaffolds with precise micro pore sizes via predictable fibre deposition. The effect of the scaffold’s architecture on cellular gene expression however has not been fully elucidated. Methods: The design and fabrication of three different uniform pore structures (250, 500 and 750 μm), as well as two offset scaffolds with different layout of fibres (30 and 50%) and one complex scaffold with three gradient pore sizes of 250–500 - 750 μm, was performed by using MEW. Calcium phosphate modification was applied to enhance the PCL scaffold hydrophilicity and bone inductivity prior to seeding with osteoblasts which were then maintained in culture for up to 30 days. Over this time, osteoblast cell morphology, matrix mineralisation, osteogenic gene expression and collagen production were assessed. Results: The in vitro findings revealed that the gradient scaffold significantly increased alkaline phosphatase activity in the attached osteoblasts while matrix mineralization was higher in the 50% offset scaffolds. The expression of osteocalcin and osteopontin genes were also upregulated compared to other osteogenic genes following 30 days culture, particularly in offset and gradient scaffold structures. Immunostaining showed significant expression of osteocalcin in offset and gradient scaffold structures. Conclusions: This study demonstrated that the heterogenous pore sizes in gradient and fibre offset PCL scaffolds prepared using MEW significantly improved the osteogenic potential of osteoblasts and hence may provide superior outcomes in bone regeneration applications.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom2: 1
dc.relation.ispartofpageto2: 16
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiomaterials Research
dc.relation.ispartofvolume24
dc.subject.fieldofresearchDentistry
dc.subject.fieldofresearchMacromolecular and materials chemistry
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchcode3203
dc.subject.fieldofresearchcode3403
dc.subject.fieldofresearchcode4003
dc.subject.keywordsBone differentiation
dc.subject.keywordsMelt electrowriting (MEW)
dc.subject.keywordsPolycaprolactone (PCL)
dc.subject.keywordsPore size
dc.subject.keywordsScaffold
dc.titleRole of offset and gradient architectures of 3-D melt electrowritten scaffold on differentiation and mineralization of osteoblasts
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAbbasi, N; Ivanovski, S; Gulati, K; Love, RM; Hamlet, S, Role of offset and gradient architectures of 3-D melt electrowritten scaffold on differentiation and mineralization of osteoblasts., Biomaterials Research, 2020, 24 (1), pp. 2: 1-2: 16
dcterms.dateAccepted2019-12-17
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-01-09T22:16:20Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s). 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorHamlet, Stephen


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