Show simple item record

dc.contributor.authorFernández-Medina, T
dc.contributor.authorVaquette, C
dc.contributor.authorIvanovski, S
dc.date.accessioned2020-01-13T02:25:19Z
dc.date.available2020-01-13T02:25:19Z
dc.date.issued2019
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms20246243
dc.identifier.urihttp://hdl.handle.net/10072/390139
dc.description.abstractHemoderivatives have utilized in an empirical manner, driven by clinical considerations, leading to the development of a plethora of manufacturing protocols. The purpose of this study was to investigate the composition and bioactivity of four common clinical-grade hemoderivates prepared using standardised methods. Four different hemoderivatives were obtained from sheep blood and divided into two groups: A-PRF/i-PRF (fresh) and P-PRP/L-PRP (anticoagulated). Thrombus (CLOT) was used as a control. Thrombocyte quantification, growth factor composition (IGF-I, VEGF, PDGF-BB, BMP-2), cell viability, migration and mineralization assay were evaluated. Platelet recovery was superior for L-PRP followed by P-PRP. A significant cumulative release of IGF-I and PDGF-BB was noted for A-PRF and L-PRP groups at early time points. Similar release profiles of BMP-2 and VEGF were noted in all protocols. Cell viability and migration assay have demonstrated a detrimental effect when the concentration was ≥60%. Moreover, at Day 21, i-PRF have demonstrated superior mineralisation properties when compared to all groups. A negative impact of A-PRF was demonstrated at high concentrations. Despite its low content in growth factors, i-PRF was the best performing blood product for inducing osteoblast mineralisation, and therefore could be the candidate of choice for utilisation in bone tissue engineering applications.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.publisher.placeSwitzerland
dc.relation.ispartofpagefrom6243: 1
dc.relation.ispartofpageto6243: 19
dc.relation.ispartofissue24
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume20
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0699
dc.subject.keywordsbone formation
dc.subject.keywordsgrowth factors
dc.subject.keywordsplatelet rich fibrin
dc.subject.keywordsplatelet rich plasma
dc.subject.keywordsstandardisation
dc.titleSystematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationFernández-Medina, T; Vaquette, C; Ivanovski, S, Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour, International Journal of Molecular Sciences, 2019, 20 (24), pp. 6243: 1-6243: 19
dcterms.dateAccepted2019-12-09
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-01-13T01:49:12Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorIvanovski, Saso


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record