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dc.contributor.authorMu, Y
dc.contributor.authorCai, P
dc.contributor.authorOlveda, RM
dc.contributor.authorRoss, AG
dc.contributor.authorOlveda, DU
dc.contributor.authorMcManus, DP
dc.date.accessioned2020-01-13T21:46:09Z
dc.date.available2020-01-13T21:46:09Z
dc.date.issued2019
dc.identifier.issn0031-1820
dc.identifier.doi10.1017/S0031182019001690
dc.identifier.urihttp://hdl.handle.net/10072/390152
dc.description.abstractNovel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase chain reaction (RT-PCR) using serum samples of S. japonicum-infected BALB/c mice. Of these, 6 miRNAs were further validated with a human cohort of individuals from a schistosomiasis-endemic area of the Philippines. RT-PCR analysis showed that two parasite-derived miRNAs (sja-miR-2b-5p and sja-miR-2c-5p) could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively. Analysis of the combined data for the two parasite miRNAs revealed a specificity of 77.4% and a sensitivity of 60.0% with an area under the curve (AUC) value of 0.6906 (P = 0.0069); however, a duplex RT-PCR targeting both sja-miR-2b-5p and sja-miR-2c-5p did not result in an increased diagnostic performance compared with the singleplex assays. Furthermore, the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five miRNAs did not. Targeting S. japonicum-derived miRNAs in serum resulted in a moderate diagnostic performance when applied to a low schistosome infection intensity setting.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCambridge University Press
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto21
dc.relation.ispartofjournalParasitology
dc.subject.fieldofresearchVeterinary Sciences
dc.subject.fieldofresearchcode0707
dc.titleParasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMu, Y; Cai, P; Olveda, RM; Ross, AG; Olveda, DU; McManus, DP, Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection, Parasitology, 2019, pp. 1-21
dc.date.updated2020-01-13T01:02:41Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered into Griffith Research Online as an Advanced Online Version.
gro.rights.copyright© 2019 Cambridge University Press. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorRoss, Allen G.


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