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dc.contributor.authorAziz, Ammar
dc.contributor.authorSarovich, Derek S
dc.contributor.authorNosworthy, Elizabeth
dc.contributor.authorBeissbarth, Jemima
dc.contributor.authorChang, Anne B
dc.contributor.authorSmith-Vaughan, Heidi
dc.contributor.authorPrice, Erin P
dc.contributor.authorHarris, Tegan M
dc.date.accessioned2020-01-14T04:24:50Z
dc.date.available2020-01-14T04:24:50Z
dc.date.issued2019
dc.identifier.issn1664-302X
dc.identifier.doi10.3389/fmicb.2019.01622
dc.identifier.urihttp://hdl.handle.net/10072/390232
dc.description.abstractNon-typeable Haemophilus influenzae (NTHi), an opportunistic pathogen of the upper airways of healthy children, can infect the lower airways, driving chronic lung disease. However, the molecular basis underpinning NTHi transition from a commensal to a pathogen is not clearly understood. Here, we performed comparative genomic and transcriptomic analyses of 12 paired, isogenic NTHi strains, isolated from the nasopharynx (NP) and bronchoalveolar lavage (BAL) of 11 children with chronic lung disease, to identify convergent molecular signatures associated with lung adaptation. Comparative genomic analyses of the 12 NP-BAL pairs demonstrated that five were genetically identical, with the remaining seven differing by only 1 to 3 mutations. Within-patient transcriptomic analyses identified between 2 and 58 differentially expressed genes in 8 of the 12 NP-BAL pairs, including pairs with no observable genomic changes. Whilst no convergence was observed at the gene level, functional enrichment analysis revealed significant under-representation of differentially expressed genes belonging to Coenzyme metabolism, Function unknown, Translation, ribosomal structure, and biogenesis Cluster of Orthologous Groups categories. In contrast, Carbohydrate transport and metabolism, Cell motility and secretion, Intracellular trafficking and secretion, and Energy production categories were over-represented. This observed trend amongst genetically unrelated NTHi strains provides evidence of convergent transcriptional adaptation of NTHi to pediatric airways that deserves further exploration. Understanding the pathoadaptative mechanisms that NTHi employs to infect and persist in the lower pediatric airways is essential for devising targeted diagnostics and treatments aimed at minimizing disease severity, and ultimately, preventing NTHi lung infections and subsequent chronic lung disease in children.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.ispartofissueJULY
dc.relation.ispartofjournalFrontiers in Microbiology
dc.relation.ispartofvolume10
dc.subject.fieldofresearchEnvironmental Science and Management
dc.subject.fieldofresearchSoil Sciences
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode0502
dc.subject.fieldofresearchcode0503
dc.subject.fieldofresearchcode0605
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsnon-typeable Haemophilus influenzae
dc.subject.keywordsNTHi
dc.titleMolecular Signatures of Non-typeable Haemophilus influenzae Lung Adaptation in Pediatric Chronic Lung Disease
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAziz, A; Sarovich, DS; Nosworthy, E; Beissbarth, J; Chang, AB; Smith-Vaughan, H; Price, EP; Harris, TM, Molecular Signatures of Non-typeable Haemophilus influenzae Lung Adaptation in Pediatric Chronic Lung Disease, Frontiers in Microbiology, 2019, 10 (JULY)
dcterms.dateAccepted2019-07-01
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-01-14T04:21:27Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 Aziz, Sarovich, Nosworthy, Beissbarth, Chang, Smith-Vaughan, Price and Harris. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorSmith-Vaughan, Heidi


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