Show simple item record

dc.contributor.authorXie, Nan
dc.contributor.authorMatigian, Nicholas
dc.contributor.authorVithanage, Tharindu
dc.contributor.authorGregory, Kye
dc.contributor.authorNassar, Zeyad D
dc.contributor.authorCabot, Peter J
dc.contributor.authorShaw, Paul N
dc.contributor.authorKirkpatrick, Carl MJ
dc.contributor.authorKim-Anh, Le Cao
dc.contributor.authorSturgess, David
dc.contributor.authorParat, Marie-Odile
dc.date.accessioned2020-01-17T02:38:04Z
dc.date.available2020-01-17T02:38:04Z
dc.date.issued2018
dc.identifier.issn1078-0432
dc.identifier.doi10.1158/1078-0432.CCR-18-0172
dc.identifier.urihttp://hdl.handle.net/10072/390578
dc.description.abstractPurpose: The purpose of this study is to investigate the potential interplay between opioid analgesia and tumor metastasis through modulation of μ-opioid receptor (MOR), Toll-like receptor 4 (TLR4) activation, and matrix degradation potential.Experimental Design: Plasma samples were collected from 60 patients undergoing elective lower limb joint replacement preoperatively and at 3, 6, and 24 hours after surgery; pain scores were documented at the same time points. Opioid administration was recorded and converted into morphine IV equivalents. Plasma samples were also collected from 10 healthy volunteers. Alphascreen cyclic AMP assay and MOR-overexpressing cells were employed to quantify MOR activation. HEK-Blue hTLR4 were utilized to measure TLR4 activation. Circulating matrix metalloprotease and tissue inhibitor of matrix protease activities were assessed by gelatin zymography and reverse zymography, respectively.Results: Postoperative plasma samples displayed the ability to activate MOR and to inhibit lipopolysaccharide (LPS)-induced TLR4 activation. Linear mixed model analysis revealed that MOR activation had a significant effect on inhibition of LPS-induced TLR4 activation. Furthermore, TLR4 had a significant effect to explain pain scores. Postoperative samples also displayed altered circulating matrix-degrading enzymes activity potential, but this was correlated neither to opioid administration nor to MOR activation potential.Conclusions: Our results show for the first time that (i) opioids administered to surgery patients result in modulation of ligand-induced TLR4 activation and (ii) postoperative pain is associated with increased circulating TLR4 activation potential. Our study further promotes the use of MOR activation potential rather than opioid intake in clinical studies measuring opioid exposure at a given time point.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Association for Cancer Research
dc.relation.ispartofpagefrom2319
dc.relation.ispartofpageto2327
dc.relation.ispartofissue10
dc.relation.ispartofjournalClinical Cancer Research
dc.relation.ispartofvolume24
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCOLON-CANCER
dc.subject.keywordsIN-VITRO
dc.titleEffect of Perioperative Opioids on Cancer-Relevant Circulating Parameters: Mu Opioid Receptor and Toll-Like Receptor 4 Activation Potential, and Proteolytic Profile
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationXie, N; Matigian, N; Vithanage, T; Gregory, K; Nassar, ZD; Cabot, PJ; Shaw, PN; Kirkpatrick, CMJ; Kim-Anh, LC; Sturgess, D; Parat, M-O, Effect of Perioperative Opioids on Cancer-Relevant Circulating Parameters: Mu Opioid Receptor and Toll-Like Receptor 4 Activation Potential, and Proteolytic Profile, Clinical Cancer Research, 2018, 24 (10), pp. 2319-2327
dcterms.dateAccepted2018-02-28
dc.date.updated2020-01-17T02:36:12Z
gro.hasfulltextNo Full Text
gro.griffith.authorVithanage, Tharindu D.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record