dc.contributor.author | Aboonabi, A | |
dc.contributor.author | Singh, I | |
dc.contributor.author | Rose' Meyer, R | |
dc.date.accessioned | 2020-02-10T01:31:42Z | |
dc.date.available | 2020-02-10T01:31:42Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0009-2797 | |
dc.identifier.doi | 10.1016/j.cbi.2020.108940 | |
dc.identifier.uri | http://hdl.handle.net/10072/391261 | |
dc.description.abstract | Type 2 diabetes is associated with oxidative stress and low-grade inflammation resulting in endothelial dysfunction (ED). This study determined to explore the protective effects of berry-derived anthocyanins (AC) with potent antioxidant and anti-inflammatory activities in human diabetic endothelial cells upon oxidative and inflammatory stressors. Cultured healthy human aortic endothelial cells (HAEC) and diabetic human aortic endothelial cells (D-HAEC) exposed to oxidative stress by hydrogen peroxide (H2O2, 75 μM) and lipopolysaccharide (LPS, 1 μg/mL) as an inflammatory inducer before treatment with AC (50 μl/ml). The results from cytotoxicity assays showed that AC had no significant effects in cell viability (P-value < 0.0001), and exposure to H2O2 75 μM had a less toxic effect (P-value < 0.05). Although, AC significantly decreased H2O2-induced cytotoxicity and oxidative stress in both HAEC and D-HAEC cell lines (P-value < 0.0001), no positive impact of AC was found on the GSSG/GSH ratios (P-value < 0.05). Exposure to the LPS increased the production of IL-6 in both HAEC and D-HAEC cell lines (P-value < 0.0001), whereas AC treatment reduced LPS‐induced IL‐6 production in both cell lines with a more robust impact on D-HAEC (P-value < 0.0001). While LPS increased inflammasome assembling and caspase-1 activation, AC treatment inhibited caspase-1 activation in D-HAEC (P ≤ 0.05). This study indicated that berry anthocyanins reduced oxidative stress and inflammation via the inhibition of the NF-ƙB signaling pathway, which contributes to mitigating the diabetes-induced up-regulation of NF-ƙB. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | Ireland | |
dc.relation.ispartofpagefrom | 108940: 1 | |
dc.relation.ispartofpageto | 108940: 10 | |
dc.relation.ispartofjournal | Chemico-Biological Interactions | |
dc.relation.ispartofvolume | 317 | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.keywords | Anthocyanins | |
dc.subject.keywords | Endothelial dysfunction | |
dc.subject.keywords | Inflammation | |
dc.subject.keywords | Oxidative stress | |
dc.subject.keywords | Type 2 diabetes mellitus | |
dc.title | Cytoprotective effects of berry anthocyanins against induced oxidative stress and inflammation in primary human diabetic aortic endothelial cells | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Aboonabi, A; Singh, I; Rose' Meyer, R, Cytoprotective effects of berry anthocyanins against induced oxidative stress and inflammation in primary human diabetic aortic endothelial cells, Chemico-Biological Interactions, 2020, 317, pp. 108940: 1-108940: 10 | |
dcterms.dateAccepted | 2020-01-08 | |
dc.date.updated | 2020-02-10T01:22:52Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Singh, Indu | |
gro.griffith.author | Aboonabi, Anahita | |
gro.griffith.author | Rose'Meyer, Roselyn B. | |