Show simple item record

dc.contributor.authorAboonabi, A
dc.contributor.authorSingh, I
dc.contributor.authorRose' Meyer, R
dc.date.accessioned2020-02-10T01:31:42Z
dc.date.available2020-02-10T01:31:42Z
dc.date.issued2020
dc.identifier.issn0009-2797
dc.identifier.doi10.1016/j.cbi.2020.108940
dc.identifier.urihttp://hdl.handle.net/10072/391261
dc.description.abstractType 2 diabetes is associated with oxidative stress and low-grade inflammation resulting in endothelial dysfunction (ED). This study determined to explore the protective effects of berry-derived anthocyanins (AC) with potent antioxidant and anti-inflammatory activities in human diabetic endothelial cells upon oxidative and inflammatory stressors. Cultured healthy human aortic endothelial cells (HAEC) and diabetic human aortic endothelial cells (D-HAEC) exposed to oxidative stress by hydrogen peroxide (H2O2, 75 μM) and lipopolysaccharide (LPS, 1 μg/mL) as an inflammatory inducer before treatment with AC (50 μl/ml). The results from cytotoxicity assays showed that AC had no significant effects in cell viability (P-value < 0.0001), and exposure to H2O2 75 μM had a less toxic effect (P-value < 0.05). Although, AC significantly decreased H2O2-induced cytotoxicity and oxidative stress in both HAEC and D-HAEC cell lines (P-value < 0.0001), no positive impact of AC was found on the GSSG/GSH ratios (P-value < 0.05). Exposure to the LPS increased the production of IL-6 in both HAEC and D-HAEC cell lines (P-value < 0.0001), whereas AC treatment reduced LPS‐induced IL‐6 production in both cell lines with a more robust impact on D-HAEC (P-value < 0.0001). While LPS increased inflammasome assembling and caspase-1 activation, AC treatment inhibited caspase-1 activation in D-HAEC (P ≤ 0.05). This study indicated that berry anthocyanins reduced oxidative stress and inflammation via the inhibition of the NF-ƙB signaling pathway, which contributes to mitigating the diabetes-induced up-regulation of NF-ƙB.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeIreland
dc.relation.ispartofpagefrom108940: 1
dc.relation.ispartofpageto108940: 10
dc.relation.ispartofjournalChemico-Biological Interactions
dc.relation.ispartofvolume317
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3101
dc.subject.keywordsAnthocyanins
dc.subject.keywordsEndothelial dysfunction
dc.subject.keywordsInflammation
dc.subject.keywordsOxidative stress
dc.subject.keywordsType 2 diabetes mellitus
dc.titleCytoprotective effects of berry anthocyanins against induced oxidative stress and inflammation in primary human diabetic aortic endothelial cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAboonabi, A; Singh, I; Rose' Meyer, R, Cytoprotective effects of berry anthocyanins against induced oxidative stress and inflammation in primary human diabetic aortic endothelial cells, Chemico-Biological Interactions, 2020, 317, pp. 108940: 1-108940: 10
dcterms.dateAccepted2020-01-08
dc.date.updated2020-02-10T01:22:52Z
gro.hasfulltextNo Full Text
gro.griffith.authorSingh, Indu
gro.griffith.authorAboonabi, Anahita
gro.griffith.authorRose'Meyer, Roselyn B.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record