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dc.contributor.authorJutras, Philippe V
dc.contributor.authorSainsbury, Frank
dc.contributor.authorGoulet, Marie-Claire
dc.contributor.authorLavoie, Pierre-Olivier
dc.contributor.authorTardif, Rachel
dc.contributor.authorHamel, Louis-Philippe
dc.contributor.authorD’Aoust, Marc-André
dc.contributor.authorMichaud, Dominique
dc.date.accessioned2020-02-11T00:27:21Z
dc.date.available2020-02-11T00:27:21Z
dc.date.issued2020
dc.identifier.issn1535-3893
dc.identifier.doi10.1021/acs.jproteome.9b00409
dc.identifier.urihttp://hdl.handle.net/10072/391298
dc.description.abstractPartial neutralization of the Golgi lumen pH by the ectopic expression of influenza virus M2 proton channel is useful to stabilize acid-labile recombinant proteins in plant cells, but the impact of pH gradient mitigation on host cellular functions has not been investigated. Here, we assessed the unintended effects of M2 expression on the leaf proteome of Nicotiana benthamiana infiltrated with the bacterial gene vector Agrobacterium tumefaciens. An isobaric tags for relative and absolute quantification quantitative proteomics procedure was followed to compare the leaf proteomes of plants agroinfiltrated with either an “empty” vector or an M2-encoding vector. Leaves infiltrated with the empty vector had a low soluble protein content compared to noninfiltrated control leaves, associated with increased levels of stress-related proteins but decreased levels of photosynthesis-associated proteins. M2 expression partly compromised these effects of agroinfiltration to restore soluble protein content in the leaf tissue, associated with restored levels of photosynthesis-associated proteins and reduced levels of stress-related proteins in the apoplast. These data illustrate the cell-wide influence of the Golgi lumen pH homeostasis on the leaf proteome of N. benthamiana responding to microbial challenge. They also underline the relevance of assessing the eventual unintended effects of accessory proteins used to modulate specific cellular or metabolic functions in plant protein biofactories.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom106
dc.relation.ispartofpageto118
dc.relation.ispartofjournalJournal of Proteome Research
dc.relation.ispartofvolume19
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode31
dc.titlepH Gradient Mitigation in the Leaf Cell Secretory Pathway Alters the Defense Response of Nicotiana benthamiana to Agroinfiltration
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationJutras, PV; Sainsbury, F; Goulet, M-C; Lavoie, P-O; Tardif, R; Hamel, L-P; D’Aoust, M-A; Michaud, D, pH Gradient Mitigation in the Leaf Cell Secretory Pathway Alters the Defense Response of Nicotiana benthamiana to Agroinfiltration, Journal of Proteome Research, 2020, 19, pp. 106-118
dc.date.updated2020-02-10T05:52:55Z
dc.description.versionSubmitted Manuscript (SM)
gro.rights.copyrightThis document is the Pre-Print: unedited Author’s version of a Published Work that appeared in final form in the Journal of Proteome Research, copyright 2020 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.9b00409
gro.hasfulltextFull Text
gro.griffith.authorSainsbury, Frank


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