Show simple item record

dc.contributor.authorThi, Thu Hao Van
dc.contributor.authorLacey, Jake A
dc.contributor.authorVezina, Ben
dc.contributor.authorPhung, Canh
dc.contributor.authorAnwar, Arif
dc.contributor.authorScott, Peter C
dc.contributor.authorMoore, Robert J
dc.date.accessioned2020-02-11T00:57:30Z
dc.date.available2020-02-11T00:57:30Z
dc.date.issued2019
dc.identifier.issn1664-302X
dc.identifier.doi10.3389/fmicb.2019.00107
dc.identifier.urihttp://hdl.handle.net/10072/391299
dc.description.abstractChickens infected with Campylobacter jejuni or Campylobacter coli are largely asymptomatic, however, infection with the closely related species, Campylobacter hepaticus, can result in Spotty Liver Disease (SLD). C. hepaticus has been detected in the liver, bile, small intestine and caecum of SLD affected chickens. The survival and colonization mechanisms that C. hepaticus uses to colonize chickens remain unknown. In this study, we compared the genome sequences of 14 newly sequenced Australian isolates of C. hepaticus, isolates from outbreaks in the United Kingdom, and reference strains of C. jejuni and C. coli, with the aim of identifying virulence genes associated with SLD. We also carried out global comparative transcriptomic analysis between C. hepaticus recovered from the bile of SLD infected chickens and C. hepaticus grown in vitro. This revealed how the bacteria adapt to proliferate in the challenging host environment in which they are found. Additionally, biochemical experiments confirmed some in silico metabolic predictions. We found that, unlike other Campylobacter sp., C. hepaticus encodes glucose and polyhydroxybutyrate metabolism pathways. This study demonstrated the metabolic plasticity of C. hepaticus, which may contribute to survival in the competitive, nutrient and energy-limited environment of the chicken. Transcriptomic analysis indicated that gene clusters associated with glucose utilization, stress response, hydrogen metabolism, and sialic acid modification may play an important role in the pathogenicity of C. hepaticus. An understanding of the survival and virulence mechanisms that C. hepaticus uses will help to direct the development of effective intervention methods to protect birds from the debilitating effects of SLD.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherFrontiers Media
dc.relation.ispartofissueFebruary
dc.relation.ispartofjournalFrontiers in Microbiology
dc.relation.ispartofvolume10
dc.subject.fieldofresearchSoil sciences
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode4106
dc.subject.fieldofresearchcode3107
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordscomparative genomics
dc.subject.keywordstranscriptomics
dc.titleSurvival Mechanisms of Campylobacter hepaticus Identified by Genomic Analysis and Comparative Transcriptomic Analysis of in vivo and in vitro Derived Bacteria
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationThi, THV; Lacey, JA; Vezina, B; Phung, C; Anwar, A; Scott, PC; Moore, RJ, Survival Mechanisms of Campylobacter hepaticus Identified by Genomic Analysis and Comparative Transcriptomic Analysis of in vivo and in vitro Derived Bacteria, Frontiers in Microbiology, 2019, 10 (Feb)
dcterms.dateAccepted2019-01-18
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-02-11T00:54:03Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 Van, Lacey, Vezina, Phung, Anwar, Scott and Moore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorVezina, Ben


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record