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dc.contributor.authorFavard, C
dc.contributor.authorChojnacki, J
dc.contributor.authorMerida, P
dc.contributor.authorYandrapalli, N
dc.contributor.authorMak, J
dc.contributor.authorEggeling, C
dc.contributor.authorMuriaux, D
dc.date.accessioned2020-02-24T00:25:50Z
dc.date.available2020-02-24T00:25:50Z
dc.date.issued2019
dc.identifier.issn2375-2548
dc.identifier.doi10.1126/sciadv.aaw8651
dc.identifier.urihttp://hdl.handle.net/10072/391800
dc.description.abstractHIV-1 Gag protein assembles at the plasma membrane of infected cells for viral particle formation. Gag targets lipids, mainly PI(4,5)P2, at the inner leaflet of this membrane. Here, we address the question whether Gag is able to trap specifically PI(4,5)P2 or other lipids during HIV-1 assembly in the host CD4+ T lymphocytes. Lipid dynamics within and away from HIV-1 assembly sites were determined using super-resolution microscopy coupled with scanning fluorescence correlation spectroscopy in living cells. Analysis of HIV-1–infected cells revealed that, upon assembly, HIV-1 is able to specifically trap PI(4,5)P2 and cholesterol, but not phosphatidylethanolamine or sphingomyelin. Furthermore, our data showed that Gag is the main driving force to restrict the mobility of PI(4,5)P2 and cholesterol at the cell plasma membrane. This is the first direct evidence highlighting that HIV-1 creates its own specific lipid environment by selectively recruiting PI(4,5)P2 and cholesterol as a membrane nanoplatform for virus assembly.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Association for the Advancement of Science
dc.relation.ispartofissue10
dc.relation.ispartofjournalScience Advances
dc.relation.ispartofvolume5
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode06
dc.subject.keywordsScience & Technology
dc.subject.keywordsMultidisciplinary Sciences
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsHUMAN-IMMUNODEFICIENCY-VIRUS
dc.subject.keywordsMURINE LEUKEMIA-VIRUS
dc.titleHIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationFavard, C; Chojnacki, J; Merida, P; Yandrapalli, N; Mak, J; Eggeling, C; Muriaux, D, HIV-1 Gag specifically restricts PI(4,5)P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly, Science Advances, 2019, 5 (10)
dcterms.dateAccepted2019-09-09
dcterms.licensehttps://creativecommons.org/licenses/by-nc/4.0/
dc.date.updated2020-02-24T00:20:41Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
gro.hasfulltextFull Text
gro.griffith.authorMak, Johnson


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