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  • YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation

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    Author(s)
    Martinez-Gil, Marta
    Goh, Kelvin GK
    Rackaityte, Elze
    Sakamoto, Chizuko
    Audrain, Bianca
    Moriel, Danilo G
    Totsika, Makrina
    Ghigo, Jean-Marc
    Schembri, Mark A
    Beloin, Christophe
    Griffith University Author(s)
    Goh, Kelvin
    Year published
    2017
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    Abstract
    Escherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT). We showed that the yeeJ gene is present in ~40% of 96 completely sequenced E. ...
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    Escherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT). We showed that the yeeJ gene is present in ~40% of 96 completely sequenced E. coli genomes and that YeeJ exists as two length variants, albeit with no detectable functional differences. We demonstrated that YeeJ promotes biofilm formation in different settings through exposition at the cell-surface. We also showed that YeeJ contains a LysM domain that interacts with peptidoglycan and thus assists its localization into the outer membrane. Additionally, we identified the Polynucleotide Phosphorylase PNPase as a repressor of yeeJ transcription. Overall, our work provides new insight into YeeJ as a member of the recently defined IAT class, and contributes to our understanding of how commensal and pathogenic E. coli colonise their environments.
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    Journal Title
    Scientific Reports
    Volume
    7
    Issue
    1
    DOI
    https://doi.org/10.1038/s41598-017-10902-0
    Copyright Statement
    © 2017 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
    Subject
    Bacteriology
    Science & Technology
    Multidisciplinary Sciences
    Science & Technology - Other Topics
    HIGH-PATHOGENICITY ISLAND
    OUTER-MEMBRANE VESICLES
    Publication URI
    http://hdl.handle.net/10072/391805
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    • Journal articles

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