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dc.contributor.authorMartinez-Gil, Marta
dc.contributor.authorGoh, Kelvin GK
dc.contributor.authorRackaityte, Elze
dc.contributor.authorSakamoto, Chizuko
dc.contributor.authorAudrain, Bianca
dc.contributor.authorMoriel, Danilo G
dc.contributor.authorTotsika, Makrina
dc.contributor.authorGhigo, Jean-Marc
dc.contributor.authorSchembri, Mark A
dc.contributor.authorBeloin, Christophe
dc.date.accessioned2020-02-24T01:12:46Z
dc.date.available2020-02-24T01:12:46Z
dc.date.issued2017
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/s41598-017-10902-0
dc.identifier.urihttp://hdl.handle.net/10072/391805
dc.description.abstractEscherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT). We showed that the yeeJ gene is present in ~40% of 96 completely sequenced E. coli genomes and that YeeJ exists as two length variants, albeit with no detectable functional differences. We demonstrated that YeeJ promotes biofilm formation in different settings through exposition at the cell-surface. We also showed that YeeJ contains a LysM domain that interacts with peptidoglycan and thus assists its localization into the outer membrane. Additionally, we identified the Polynucleotide Phosphorylase PNPase as a repressor of yeeJ transcription. Overall, our work provides new insight into YeeJ as a member of the recently defined IAT class, and contributes to our understanding of how commensal and pathogenic E. coli colonise their environments.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofissue1
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume7
dc.subject.fieldofresearchBacteriology
dc.subject.fieldofresearchcode310701
dc.subject.keywordsScience & Technology
dc.subject.keywordsMultidisciplinary Sciences
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsHIGH-PATHOGENICITY ISLAND
dc.subject.keywordsOUTER-MEMBRANE VESICLES
dc.titleYeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMartinez-Gil, M; Goh, KGK; Rackaityte, E; Sakamoto, C; Audrain, B; Moriel, DG; Totsika, M; Ghigo, J-M; Schembri, MA; Beloin, C, YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation, Scientific Reports, 2017, 7 (1)
dcterms.dateAccepted2017-08-16
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-02-24T01:08:50Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2017 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorGoh, Kelvin


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