Show simple item record

dc.contributor.authorDeveryshetty, Jaigeethen_US
dc.contributor.authorS. Phale, Prashanten_US
dc.contributor.editorH L Drakeen_US
dc.date.accessioned2017-05-03T15:52:58Z
dc.date.available2017-05-03T15:52:58Z
dc.date.issued2009en_US
dc.date.modified2011-06-21T07:56:25Z
dc.identifier.issn1465-2080en_US
dc.identifier.doi10.1099/mic.0.030460-0en_AU
dc.identifier.urihttp://hdl.handle.net/10072/39184
dc.description.abstractPseudomonas sp. strain PPD can metabolize phenanthrene as the sole source of carbon and energy via the 'phthalic acid' route. The key enzyme, 1-hydroxy-2-naphthoic acid dioxygenase (1-HNDO, EC 1.13.11.38), was purified to homogeneity using a 3-hydroxy-2-naphthoic acid (3-H2NA)-affinity matrix. The enzyme was a homotetramer with a native molecular mass of 160 kDa and subunit molecular mass of ~39 kDa. It required Fe(II) as the cofactor and was specific for 1-hydroxy-2-naphthoic acid (1-H2NA), with Km 13.5 占and Vmax 114?孯l min-1 mg-1. 1-HNDO failed to show activity with gentisic acid, salicylic acid and other hydroxynaphthoic acids tested. Interestingly, the enzyme showed substrate inhibition with a Ki of 116 卮 1-HNDO was found to be competitively inhibited by 3-H2NA with a Ki of 24 卮 Based on the pH-dependent spectral changes, the enzyme reaction product was identified as 2-carboxybenzalpyruvic acid. Under anaerobic conditions, the enzyme failed to convert 1-H2NA to 2-carboxybenzalpyruvic acid. Stoichiometric studies showed the incorporation of 1 mol O2 into the substrate to yield 1 mol product. These results suggest that 1-HNDO from Pseudomonas sp. strain PPD is an extradiol-type ring-cleaving dioxygenase.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherSociety for General Microbiologyen_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom3083en_US
dc.relation.ispartofpageto3091en_US
dc.relation.ispartofissue9en_US
dc.relation.ispartofjournalMicrobiologyen_US
dc.relation.ispartofvolume155en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchStructural Biology (incl. Macromolecular Modelling)en_US
dc.subject.fieldofresearchcode060112en_US
dc.titleBiodegradation of phenanthrene by Pseudomonas sp. strain PPD: purification and characterization of 1-hydroxy-2-naphthoic acid dioxygenaseen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2009
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record