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dc.contributor.authorMeinert, Christoph
dc.contributor.authorSchrobback, Karsten
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorKlein, Travis J
dc.date.accessioned2020-02-26T23:43:45Z
dc.date.available2020-02-26T23:43:45Z
dc.date.issued2017
dc.identifier.issn2045-2322en_US
dc.identifier.doi10.1038/s41598-017-16523-xen_US
dc.identifier.urihttp://hdl.handle.net/10072/391940
dc.description.abstractThe ex vivo engineering of autologous cartilage tissues has the potential to revolutionize the clinical management of joint disorders. Yet, high manufacturing costs and variable outcomes associated with tissue-engineered implants are still limiting their application. To improve clinical outcomes and facilitate a wider use of engineered tissues, automated bioreactor systems capable of enhancing and monitoring neotissues are required. Here, we developed an innovative system capable of applying precise uni- or biaxial mechanical stimulation to developing cartilage neotissues in a tightly controlled and automated fashion. The bioreactor allows for simple control over the loading parameters with a user-friendly graphical interface and is equipped with a load cell for monitoring tissue maturation. Applying our bioreactor, we demonstrate that human articular chondrocytes encapsulated in hydrogels composed of gelatin methacryloyl (GelMA) and hyaluronic acid methacrylate (HAMA) respond to uni- and biaxial mechanical stimulation by upregulation of hyaline cartilage-specific marker genes. We further demonstrate that intermittent biaxial mechanostimulation enhances accumulation of hyaline cartilage-specific extracellular matrix. Our study underlines the stimulatory effects of mechanical loading on the biosynthetic activity of human chondrocytes in engineered constructs and the need for easy-to-use, automated bioreactor systems in cartilage tissue engineering.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.language.isoeng
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalScientific Reportsen_US
dc.relation.ispartofvolume7en_US
dc.subject.fieldofresearchBiochemistry and Cell Biologyen_US
dc.subject.fieldofresearchOther Physical Sciencesen_US
dc.subject.fieldofresearchcode0601en_US
dc.subject.fieldofresearchcode0299en_US
dc.subject.keywordsScience & Technologyen_US
dc.subject.keywordsMultidisciplinary Sciencesen_US
dc.subject.keywordsScience & Technology - Other Topicsen_US
dc.subject.keywordsARTICULAR-CARTILAGEen_US
dc.subject.keywordsHYDROSTATIC-PRESSUREen_US
dc.titleA novel bioreactor system for biaxial mechanical loading enhances the properties of tissue-engineered human cartilageen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationMeinert, C; Schrobback, K; Hutmacher, DW; Klein, TJ, A novel bioreactor system for biaxial mechanical loading enhances the properties of tissue-engineered human cartilage, Scientific Reports, 2017, 7 (1)en_US
dcterms.dateAccepted2017-11-13
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2020-02-26T23:40:56Z
dc.description.versionVersion of Record (VoR)en_US
gro.rights.copyright© The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
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gro.griffith.authorHutmacher, Dietmar W.


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