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dc.contributor.authorChhaya, Mohit P
dc.contributor.authorBalmayor, Elizabeth R
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorSchantz, Jan-Thorsten
dc.date.accessioned2020-02-27T00:32:02Z
dc.date.available2020-02-27T00:32:02Z
dc.date.issued2016
dc.identifier.issn2045-2322en_US
dc.identifier.doi10.1038/srep28030en_US
dc.identifier.urihttp://hdl.handle.net/10072/391943
dc.description.abstractAdipose tissue engineering offers a promising alternative to current breast reconstruction options. However, the conventional approach of using a scaffold in combination with adipose-derived precursor cells poses several problems in terms of scalability and hence clinical feasibility. Following the body-as-a-bioreactor approach, this study proposes a unique concept of delayed fat injection into an additive biomanufactured and custom-made scaffold. Three study groups were evaluated: Empty scaffold, Scaffold containing 4 cm3 lipoaspirate and Empty scaffold +2-week prevascularisation period. In group 3, of prevascularisation, 4 cm3 of lipoaspirate was injected into scaffolds after 2 weeks. Using a well-characterised additive biomanufacturing technology platform, patient-specific scaffolds made of medical-grade-polycaprolactone were designed and fabricated. Scaffolds were implanted in subglandular pockets in immunocompetent minipigs (n = 4) for 24-weeks. Angiogenesis and adipose tissue regeneration were observed in all constructs. Histological evaluation showed that the prevascularisation + lipoaspirate group had the highest relative area of adipose tissue (47.32% ± 4.12) which was significantly higher than both lipoaspirate-only (39.67% ± 2.04) and empty control group (8.31% ± 8.94) and similar to native breast tissue (44.97% ± 14.12). This large preclinical animal study provides proof-of-principle that the clinically applicable prevascularisation and delayed fat-injection techniques can be used for regeneration of large volumes of adipose tissue.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.language.isoeng
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalScientific Reportsen_US
dc.relation.ispartofvolume6en_US
dc.subject.fieldofresearchBiochemistry and Cell Biologyen_US
dc.subject.fieldofresearchOther Physical Sciencesen_US
dc.subject.fieldofresearchcode0601en_US
dc.subject.fieldofresearchcode0299en_US
dc.subject.keywordsScience & Technologyen_US
dc.subject.keywordsMultidisciplinary Sciencesen_US
dc.subject.keywordsScience & Technology - Other Topicsen_US
dc.subject.keywordsADIPOSE-TISSUEen_US
dc.subject.keywordsENDOTHELIAL-CELLSen_US
dc.titleTransformation of Breast Reconstruction via Additive Biomanufacturingen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationChhaya, MP; Balmayor, ER; Hutmacher, DW; Schantz, J-T, Transformation of Breast Reconstruction via Additive Biomanufacturing, Scientific Reports, 2016, 6 (1)en_US
dcterms.dateAccepted2016-05-23
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2020-02-27T00:29:01Z
dc.description.versionVersion of Record (VoR)en_US
gro.rights.copyright© The Author(s) 2016. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
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gro.griffith.authorHutmacher, Dietmar W.


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