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dc.contributor.authorWan, Zhengfen
dc.contributor.authorUmer, Muhammad
dc.contributor.authorLobino, Mirko
dc.contributor.authorThiel, David
dc.contributor.authorNam-Trung, Nguyen
dc.contributor.authorTrinchi, Adrian
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorGao, Yongsheng
dc.contributor.authorLi, Qin
dc.date.accessioned2020-03-25T03:11:26Z
dc.date.available2020-03-25T03:11:26Z
dc.date.issued2020
dc.identifier.issn0008-6223
dc.identifier.doi10.1016/j.carbon.2020.03.043
dc.identifier.urihttp://hdl.handle.net/10072/392615
dc.description.abstractWe report a sensitive, yet low-cost biosensor based on laser induced graphene for femtomolar microRNA (miRNA) detection. Combined with the miRNA extraction and magnetic isolation process, the target miRNAs were purified for further detection using laser induced graphene sensor. The laser induced graphene was prepared by direct laser writing on commercial polyimide (PI) and patterned via a computer-aided design system as an electrode for electrochemical biosensing. We found that the laser reduction of PI resulted in nitrogen-doped porous graphene, not only improving its conductivity but also its sensitivity to nucleic acids. Preeclampsia specific miRNA hsa-miR-486-5p was magnetically purified and directly adsorbed on the surface of graphene electrode via graphene-miRNA affinity interaction. Surface attached miRNAs were then electrochemically quantified using [Fe(CN)6]3-/4- redox system. Our assay demonstrates detection of miRNA has-miR-486-5p up to concentrations as low as 10 fM with excellent reproducibility. Owing to its facile fabrication, low cost and high performance, the laser induced N-doped graphene biosensor presented here shows great potential for applications in detecting miRNA in biomedical applications.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofjournalCarbon
dc.subject.fieldofresearchPhysical sciences
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchcode51
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode40
dc.titleLaser induced self-N-doped porous graphene as an electrochemical biosensor for femtomolar miRNA detection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationWan, Z; Umer, M; Lobino, M; Thiel, D; Nguyen, N-T; Trinchi, A; Shiddiky, MJA; Gao, Y; Li, Q, Laser induced self-N-doped porous graphene as an electrochemical biosensor for femtomolar miRNA detection, Carbon, 2020
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2020-03-25T01:05:47Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2020 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorUmer, Muhammad
gro.griffith.authorLi, Qin
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorWan, Zhengfen
gro.griffith.authorGao, Yongsheng
gro.griffith.authorThiel, David V.
gro.griffith.authorNguyen, Nam-Trung


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