Enhanced Tissue Damage Following H1N1 Infection in Human Precision-Cut Lung Slices (PCLS)

Danov, O; Martin, G; Greif, A; Bailly, B; Braubach, P; Jonigk, DD; Warnecke, G; von Itzstein, M; Sewald, K; Wronski, S; Braun, A

doi:10.1164/ajrccm-conference.2019.199.1_MeetingAbstracts.A5742

Author(s)

Danov, O

Martin, G

Greif, A

Bailly, B

Braubach, P

Jonigk, DD

Warnecke, G

von Itzstein, M

Sewald, K

Wronski, S

Braun, A

Griffith University Author(s)

von Itzstein, Mark

Year published

2019

Metadata

Show full item record

Abstract

Annual seasonal epidemics with Influenza A virus (IAV) strains result in 3-5 million severe cases in children and elderly individuals. Therefore, in this study the immune response of lower respiratory tract tissue to H1N1 (California/04/2009) pandemic strain was compared to seasonal H3N2 (Perth/16/2009) in viable human precision-cut lung slices (PCLS) ex vivo. PCLS were inoculated with 104-106 ffu/mL for 1 h and incubated 48 h post infection. Local immune response was measured with MSD, tissue damage observed by LDH release andAnnual seasonal epidemics with Influenza A virus (IAV) strains result in 3-5 million severe cases in children and elderly individuals. Therefore, in this study the immune response of lower respiratory tract tissue to H1N1 (California/04/2009) pandemic strain was compared to seasonal H3N2 (Perth/16/2009) in viable human precision-cut lung slices (PCLS) ex vivo. PCLS were inoculated with 104-106 ffu/mL for 1 h and incubated 48 h post infection. Local immune response was measured with MSD, tissue damage observed by LDH release and
View less >