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dc.contributor.authorWailan, Alexander M
dc.contributor.authorSartor, Anna L
dc.contributor.authorZowawi, Hosam M
dc.contributor.authorPerry, John D
dc.contributor.authorPaterson, David L
dc.contributor.authorSidjabat, Hanna E
dc.date.accessioned2020-03-29T22:24:29Z
dc.date.available2020-03-29T22:24:29Z
dc.date.issued2015
dc.identifier.issn0066-4804
dc.identifier.doi10.1128/aac.01319-15
dc.identifier.urihttp://hdl.handle.net/10072/392717
dc.description.abstractThe carbapenem resistance determinant blaNDM-1 has been found in various Gram-negative bacteria and upon different plasmid replicon types (Inc). Here, we present four patients within two hospitals in Pakistan harboring between two and four NDM-1-producing Gram-negative bacilli of different species coresident in their stool samples. We characterize the blaNDM-1 genetic contexts of these 11 NDM-1-producing Gram-negative bacilli in addition to other antimicrobial resistance mechanisms, plasmid replicon profiles, and sequence types (STs) in order to understand the underlying acquisition mechanisms of carbapenem resistance within these bacteria. Two common plasmid types (IncN2 and IncA/C) were identified to carry blaNDM-1 among the six different bacterial species isolated from the four patients. Two of these strains were novel Citrobacter freundii ST 20 and ST 21. The same IncN2-type blaNDM-1 genetic context was found in all four patients and within four different species. The IncA/C-type blaNDM-1 genetic context was found in two different species and in two of the four patients. Combining genetic context characterization with other molecular epidemiology methods, we were able to establish the molecular epidemiological links between genetically unrelated bacterial species by linking their acquisition of an IncN2 or IncA/C plasmid carrying blaNDM-1 for carbapenem resistance. By combining plasmid characterization and in-depth genetic context assessment, this analysis highlights the importance of plasmids in antimicrobial resistance. It also provides a novel approach for investigating the underlying mechanisms of blaNDM-1-related spread between bacterial species and genera via plasmids.
dc.languageEnglish
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofpagefrom7405
dc.relation.ispartofpageto7410
dc.relation.ispartofissue12
dc.relation.ispartofjournalAntimicrobial Agents and Chemotherapy
dc.relation.ispartofvolume59
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.subject.fieldofresearchcode1115
dc.titleGenetic Contexts ofblaNDM-1in Patients Carrying Multiple NDM-Producing Strains
dc.typeJournal article
dcterms.bibliographicCitationWailan, AM; Sartor, AL; Zowawi, HM; Perry, JD; Paterson, DL; Sidjabat, HE, Genetic Contexts ofblaNDM-1in Patients Carrying Multiple NDM-Producing Strains, Antimicrobial Agents and Chemotherapy, 2015, 59 (12), pp. 7405-7410
dc.date.updated2020-03-27T06:31:33Z
dc.description.versionPublished
gro.rights.copyright© 2015 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
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gro.griffith.authorSidjabat, Hanna E.


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