Show simple item record

dc.contributor.authorCox, Amanda J
dc.contributor.authorMakino, Hiroshi
dc.contributor.authorCripps, Allan W
dc.contributor.authorWest, Nicholas P
dc.date.accessioned2020-03-30T23:52:01Z
dc.date.available2020-03-30T23:52:01Z
dc.date.issued2019
dc.identifier.issn0964-7058
dc.identifier.doi10.6133/apjcn.201912_28(4).0009
dc.identifier.urihttp://hdl.handle.net/10072/392782
dc.description.abstractBackground and Objectives: A key measure for classifying bacteria as a probiotic is the ability to survive gastric transport and be recoverable in faeces. The aim of this study was to determine whether Lactobacillus casei strain Shirota (LcS) could be recovered in the faeces of healthy young Australian adults following ingestion of a fermented milk drink. Methods and Study Design: A cohort of 25 healthy individuals (male/female: 14/11; age: 29.3+-6.6 years; BMI: 25.3+-2.7 kg/m2, mean+-SD) ingested one 65 ml bottle of fermented milk containing 6.5 109 LcS live cells daily for 14 days. Participants provided a faecal sample at day 0, day 7 (mid-supplementation), day 14 (end of supplementation) and 14 days after cessation of the supplement (day 28) for assessment of the number of viable LcS via microbial culture on selective media with confirmation using a colony-direct polymerase chain reaction and species-specific primers. Results: The supplement was well tolerated by participants. No LcS colonies were recovered from participants prior to ingestion of the fermented milk drink. All participants had recoverable LcS colonies at day 7 and day 14, with a mean recovery of 6.5+-1.1 and 6.4+-1.1 log10 CFU/g of faeces (mean+-SD) at each time point respectively. LcS was detectable in only one sample at 14 days following the cessation of supplementation. Conclusions: Live LcS is recoverable in faeces from healthy Australian adults following daily ingestion of a fermented milk drink.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherHEC Press
dc.relation.ispartofpagefrom734
dc.relation.ispartofpageto739
dc.relation.ispartofissue4
dc.relation.ispartofjournalAsia Pacific Journal of Clinical Nutrition
dc.relation.ispartofvolume28
dc.subject.fieldofresearchNutrition and Dietetics
dc.subject.fieldofresearchcode1111
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsLactobacillus casei strain Shirota
dc.subject.keywordsprobiotics
dc.titleRecovery of Lactobacillus casei strain Shirota (LcS) from faeces with 14 days of fermented milk supplementation in healthy Australian adults
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationCox, AJ; Makino, H; Cripps, AW; West, NP, Recovery of Lactobacillus casei strain Shirota (LcS) from faeces with 14 days of fermented milk supplementation in healthy Australian adults, Asia Pacific Journal of Clinical Nutrition, 2019, 28 (4), pp. 734-739
dc.date.updated2020-03-30T23:45:57Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 HEC Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorCripps, Allan W.
gro.griffith.authorCox, Amanda J.
gro.griffith.authorWest, Nic P.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record