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dc.contributor.authorManer, Jenny
dc.contributor.authorBurkard, Michael
dc.contributor.authorCassano, Juan Carlos
dc.contributor.authorNash, Susan M Bengtson
dc.contributor.authorSchirmer, Kristin
dc.contributor.authorSuter, Marc J-F
dc.date.accessioned2020-04-16T22:44:36Z
dc.date.available2020-04-16T22:44:36Z
dc.date.issued2019
dc.identifier.issn2046-2069
dc.identifier.doi10.1039/c9ra05352b
dc.identifier.urihttp://hdl.handle.net/10072/393155
dc.description.abstractHumpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were discernible. Yet, a significant increase in DNA damage, detected in the alkaline comet assay, was found in HuWa cells exposed to 10 μg L−1 HCB during the sensitive phase of cell attachment. Collectively, this work provides a ready-to-use passive dosing system and delivers evidence that HCB elicits genotoxicity in humpback whale cells.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofpagefrom39447
dc.relation.ispartofpageto39457
dc.relation.ispartofissue67
dc.relation.ispartofjournalRSC Advances
dc.relation.ispartofvolume9
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchcode03
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsChemistry, Multidisciplinary
dc.subject.keywordsChemistry
dc.subject.keywordsPERSISTENT ORGANIC POLLUTANTS
dc.titleHexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationManer, J; Burkard, M; Cassano, JC; Nash, SMB; Schirmer, K; Suter, MJ-F, Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions, RSC Advances, 2019, 9 (67), pp. 39447-39457
dcterms.licensehttp://creativecommons.org/licenses/by-nc/3.0/
dc.date.updated2020-04-16T22:40:11Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorBengtson Nash, Susan
gro.griffith.authorBurkard, Michael


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