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  • Isolation and Identification of 3-Chloropropionic Acid Degrading Bacterium from Oxley Creek, Queensland, Australia

    Author(s)
    Hadi, Ahmad
    Greene, Anthony
    Griffith University Author(s)
    Greene, Tony C.
    Year published
    2019
    Metadata
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    Abstract
    This study investigated the isolation and identification of a bacterial strain that could degrade 3-chloropropionic acid (3CP) as its sole carbon source. A 3CP degrading bacteria was successfully isolated from Oxley Creek, Queensland, Australia and designated as strain O1. Analysis of 16S rRNA as well as biochemical and morphological tests, found that strain O1 shared a sequence identity and characteristics most similar to that of Lysinibacillus fusiformis. The bacteria grew at 30°C in minimal media at a maximum concentration of 20 mM 3CP, however, exhibited a rather slow doubling time of 92 hours. Most importantly, analysis ...
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    This study investigated the isolation and identification of a bacterial strain that could degrade 3-chloropropionic acid (3CP) as its sole carbon source. A 3CP degrading bacteria was successfully isolated from Oxley Creek, Queensland, Australia and designated as strain O1. Analysis of 16S rRNA as well as biochemical and morphological tests, found that strain O1 shared a sequence identity and characteristics most similar to that of Lysinibacillus fusiformis. The bacteria grew at 30°C in minimal media at a maximum concentration of 20 mM 3CP, however, exhibited a rather slow doubling time of 92 hours. Most importantly, analysis by high-performance chromatography (HPLC) revealed that the Lysinibacillus fusiformis isolate effectively degraded ∼100% of available 20mM 3CP. This is the first report detailing a Lysinibacillus fusiformis strain able to utilize 3CP as its sole carbon source competently.
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    Conference Title
    Proceedings of the 2nd International Conference on Biosciences and Medical Engineering (ICBME2019): Towards Innovative Research and Cross-Disciplinary Collaborations
    Volume
    2155
    Issue
    1
    DOI
    https://doi.org/10.1063/1.5125536
    Subject
    Microbiology
    Science & Technology
    Life Sciences & Biomedicine
    Technology
    Biology
    Engineering, Biomedical
    Publication URI
    http://hdl.handle.net/10072/393323
    Collection
    • Conference outputs

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