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dc.contributor.authorBazargan, Maryam
dc.contributor.authorDavey, Andrew K
dc.contributor.authorMuhlhausler, Beverly S
dc.contributor.authorMorrison, Janna L
dc.contributor.authorMcMillen, I Caroline
dc.contributor.authorFoster, David JR
dc.date.accessioned2017-05-03T16:01:05Z
dc.date.available2017-05-03T16:01:05Z
dc.date.issued2011
dc.date.modified2011-07-01T06:57:46Z
dc.identifier.issn0731-7085
dc.identifier.doi10.1016/j.jpba.2011.01.026
dc.identifier.urihttp://hdl.handle.net/10072/39343
dc.description.abstractThis paper describes the development of a sensitive high performance liquid chromatography (HPLC) method for quantification of rosiglitazone in sheep plasma and amniotic fluid. Samples were prepared by liquid-liquid extraction using tert-butyl methyl ether, and rosiglitazone was quantitated by HPLC using a C18 column and fluorescence detector with an excitation wavelength of 247 nm and emission wavelength of 367 nm. The mobile phase consisted of ammonium acetate (10 mM, pH 5.2) and acetonitrile (56.5:43.5, v/v) with a flow rate of 1 ml/min. Ketoconazole was used as the internal standard (IS). The plasma calibration curve was linear over the range of 2.5-250 ng/ml (mean r2 = 0.9940 ᠰ.0024; n = 6) with accuracy of 99.4-102.8% over the calibration range. The intra-day and inter-day coefficient of variation (%CV, percent coefficient of variation) were in the range of 0.01-8.68% in sheep plasma. Similar performance was achieved for amniotic fluid. The described method was successfully applied to quantitate rosiglitazone concentrations in the pregnant ewe and her fetus.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom360
dc.relation.ispartofpageto365
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Pharmaceutical and Biomedical Analysis
dc.relation.ispartofvolume55
dc.rights.retentionY
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchPharmaceutical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3214
dc.subject.fieldofresearchcode321405
dc.titleSimple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorDavey, Andrew


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