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dc.contributor.authorHifnawy, Mohammed S
dc.contributor.authorAboseada, Mahmoud A
dc.contributor.authorHassan, Hossam M
dc.contributor.authorAboulMagd, Asmaa M
dc.contributor.authorTohamy, Adel F
dc.contributor.authorAbdel-Kawi, Samraa H
dc.contributor.authorRateb, Mostafa E
dc.contributor.authorEl Naggar, El Moataz Bellah
dc.contributor.authorLiu, Miaomiao
dc.contributor.authorQuinn, Ronald J
dc.contributor.authorAlhadrami, Hani A
dc.contributor.authorAbdelmohsen, Usama Ramadan
dc.date.accessioned2020-05-05T00:38:18Z
dc.date.available2020-05-05T00:38:18Z
dc.date.issued2020
dc.identifier.issn2218-1989
dc.identifier.doi10.3390/metabo10010031
dc.identifier.urihttp://hdl.handle.net/10072/393574
dc.description.abstractMany routes have been explored to search for effective, safe, and affordable alternatives to hazardous female contraceptives. Herbal extracts and their secondary metabolites are some of the interesting research areas to address this growing issue. This study aims to investigate the effects of ten different plant extracts on testicular spermatogenesis. The correlation between the chemical profile of these extracts and their in vivo effect on male reproductive system was evaluated using various techniques. Approximately 10% of LD50 of hydro-methanolic extracts were orally administrated to rats for 60 days. Semen parameters, sexual organ weights, and serum levels of male sex hormones in addition to testes histopathology, were evaluated. Moreover, metabolomic analysis using (LC-HRESIMS), multivariate analysis (PCA), immunohistochemistry (caspase-3 and β-catenin), and a docking study were performed. Results indicated that three plant extracts significantly decreased epididymal sperm density and motility. Moreover, their effects on testicular cells were also assured by histopathological evaluations. Metabolomic profiling of the bioactive plant extracts showed the presence of diverse phytochemicals, mostly oleanane saponins, phenolic diterpenes, and lupane triterpenes. A docking study on caspase-3 enzyme showed that oleanane saponins possessed the highest binding affinity. An immunohistochemistry assay on β-catenin and caspase-3 indicated that Albizzia lebbeck was the most active extract for decreasing immunoexpression of β-catenin, while Rosmarinus officinalis showed the highest activity for increasing immunoexpression of caspase-3. The spermatogenesis decreasing the activity of A. lebbeck, Anagallis arvensis, and R. officinalis can be mediated via up-regulation of caspase-3 and down-regulation of β-catenin existing in testis cells. View Full-Text
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofpagefrom31:1
dc.relation.ispartofpageto31:23
dc.relation.ispartofissue1
dc.relation.ispartofjournalMetabolites
dc.relation.ispartofvolume10
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3205
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordstesticular spermatogenesis
dc.subject.keywordsbeta-catenin
dc.titleTesticular Caspase-3 and beta-Catenin Regulators Predicted via Comparative Metabolomics and Docking Studies
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHifnawy, MS; Aboseada, MA; Hassan, HM; AboulMagd, AM; Tohamy, AF; Abdel-Kawi, SH; Rateb, ME; El Naggar, EMB; Liu, M; Quinn, RJ; Alhadrami, HA; Abdelmohsen, UR, Testicular Caspase-3 and beta-Catenin Regulators Predicted via Comparative Metabolomics and Docking Studies, Metabolites, 2020, 10 (1), pp. 31:1-31:23
dcterms.dateAccepted2020-01-06
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-05-05T00:31:38Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLiu, Miaomiao
gro.griffith.authorQuinn, Ronald J.


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