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dc.contributor.authorHofstee, Pierre
dc.contributor.authorCuffe, James SM
dc.contributor.authorPerkins, Anthony V
dc.date.accessioned2020-05-06T03:54:18Z
dc.date.available2020-05-06T03:54:18Z
dc.date.issued2020
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms21062210
dc.identifier.urihttp://hdl.handle.net/10072/393625
dc.description.abstractThe human selenoproteome is comprised of ~25 genes, which incorporate selenium, in the form of selenocysteine, into their structure. Since it is well known that selenium is important to maternal health and foetal development during pregnancy, this study aimed at defining the impact of selenium deficiency on maternal, placental, foetal and offspring selenoprotein gene expression. Female C57BL/6 mice were randomly allocated to control (>190 μg/kg) or low selenium (<50 μg/kg) diets four weeks prior to mating and throughout gestation. At embryonic day (E)18.5, pregnant mice were sacrificed followed by collection of maternal and foetal tissues. A subset of mice littered down, and offspring were monitored from postnatal day (PN) 8, weaned at PN24 and sacrificed at PN180, followed by tissue collection. Following RNA extraction, the expression of 14 selenoproteins was assessed with qPCR in liver, kidneys, muscle and placenta. Selenium deficiency downregulated expression (Ptrt < 0.05) of many selenoproteins in maternal tissues and the placenta. However, foetal selenoprotein expression was upregulated (Ptrt < 0.05) in all tissues, especially the kidneys. This was not reflected at PN180; however, a sexually dimorphic relationship in selenoprotein expression was observed in offspring. This study demonstrates the selenoproteome is sensitive to dietary selenium levels, which may be exacerbated by pregnancy. We concluded that transcriptional regulation of selenoproteins is complex and multifaceted, with expression exhibiting tissue-, age-and sex-specificities.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom2210
dc.relation.ispartofpageto2210
dc.relation.ispartofissue6
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume21
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther biological sciences
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3199
dc.subject.keywordsDOHaD
dc.subject.keywordsgene expression
dc.subject.keywordsnutrients
dc.subject.keywordspregnancy
dc.subject.keywordsreproduction
dc.titleAnalysis of selenoprotein expression in response to dietary selenium deficiency during pregnancy indicates tissue specific differential expression in mothers and sex specific changes in the fetus and offspring
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHofstee, P; Cuffe, JSM; Perkins, AV, Analysis of selenoprotein expression in response to dietary selenium deficiency during pregnancy indicates tissue specific differential expression in mothers and sex specific changes in the fetus and offspring, International Journal of Molecular Sciences, 2020, 21 (6), pp. 2210-2210
dcterms.dateAccepted2020-03-17
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-05-06T03:13:49Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorPerkins, Anthony V.
gro.griffith.authorHofstee, Pierre D.


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