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dc.contributor.authorIslam, F
dc.contributor.authorGopalan, V
dc.contributor.authorLam, AK
dc.description.abstractCancer stem cells (CSCs) are a small subpopulation of cells associated with cancer initiation, progression, metastasis, therapy resistant, and recurrence. In esophageal squamous cell carcinoma (ESCC), several cell surface and intracellular markers, for example, CD44, ALDH, Pygo2, MAML1, Twist1, Musashi1, side population (SP), CD271, and CD90, have been proposed to identify CSCs. In addition, stem cell markers such as ALDH1, HIWI, Oct3/4, ABCG2, SOX2, SALL4, BMI-1, NANOG, CD133, and podoplanin were associated with pathological stages of cancer, cancer recurrence, prognosis, and therapy resistance of patients with ESCC. Identification and isolation of CSCs could play an important part of improved cancer management regime in ESCC. Furthermore, CSCs may be used as the predictive tool for chemoradiotherapy response in ESCC. Different methods such as in vitro functional assays, cell sorting using various intracellular, and cell surface markers and xenotransplantation techniques are frequently used for the identification and isolation of CSCs in different cancers, including ESCC. However, none of these methods solely can guarantee complete isolation of CSC population. Therefore, a combination of methods is used for reliable detection and isolation of CSCs. Herein, we describe the identification and isolation of CSCs from ESCC cells by cell sorting after Hoechst 33342 staining followed by in vitro functional assays and in vivo mouse xenotransplantation techniques.
dc.publisherSpringer US
dc.relation.ispartofjournalMethods in Molecular Biology
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.keywordsCSC identification
dc.subject.keywordsCSC isolation
dc.subject.keywordsCancer stem cell
dc.subject.keywordsEsophageal squamous cell carcinoma
dc.subject.keywordsIn vitro assays
dc.titleDetention and Identification of Cancer Stem Cells in Esophageal Squamous Cell Carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationIslam, F; Gopalan, V; Lam, AK, Detention and Identification of Cancer Stem Cells in Esophageal Squamous Cell Carcinoma, Methods in Molecular Biology, 2020, 2129, pp. 177-191
gro.hasfulltextNo Full Text
gro.griffith.authorGopalan, Vinod
gro.griffith.authorLam, Alfred K.
gro.griffith.authorIslam, Farhad

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