Show simple item record

dc.contributor.authorIslam, F
dc.contributor.authorGopalan, V
dc.contributor.authorLam, AK
dc.date.accessioned2020-05-08T01:44:12Z
dc.date.available2020-05-08T01:44:12Z
dc.date.issued2020
dc.identifier.issn1064-3745
dc.identifier.doi10.1007/978-1-0716-0377-2_20
dc.identifier.urihttp://hdl.handle.net/10072/393692
dc.description.abstractTumor-associated antigens (TAAs) can be used as cancer markers and as signposts of therapeutic targets since their inimitable expression in cancer or significant overexpression in esophageal squamous cell carcinoma (ESCC) correlates with the initiation and progression of the diseases. Immunoblotting, also known as Western blotting or protein blotting, is a core technique in cell and molecular biology to detect proteins and glycoproteins. The technique allows detection of TAAs from complex protein samples such as in serum, aspirate, or solid tumor homogenate. In the process, proteins are separated according to the molecular weight. They were visualized within a gel matrix and then transferred to a supporting membrane. Finally, they are probed for binding with corresponding antibodies and identified the target proteins. Herein, we describe the Western blots analysis to detect protein or glycoprotein in samples from patients with esophageal squamous cell carcinoma (ESCC) or cells derived from ESCC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer US
dc.relation.ispartofpagefrom269
dc.relation.ispartofpageto277
dc.relation.ispartofjournalMethods in Molecular Biology
dc.relation.ispartofvolume2129
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3404
dc.subject.keywordsESCC
dc.subject.keywordsImmunoblots
dc.subject.keywordsProtein blots
dc.subject.keywordsTumor-associated antigen
dc.subject.keywordsWestern blots
dc.titleImmunoblotting in Detection of Tumor-Associated Antigens in Esophageal Squamous Cell Carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationIslam, F; Gopalan, V; Lam, AK, Immunoblotting in Detection of Tumor-Associated Antigens in Esophageal Squamous Cell Carcinoma, Methods in Molecular Biology, 2020, 2129, pp. 269-277
dc.date.updated2020-05-08T01:39:53Z
gro.hasfulltextNo Full Text
gro.griffith.authorGopalan, Vinod
gro.griffith.authorLam, Alfred K.
gro.griffith.authorIslam, Farhad


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record