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dc.contributor.authorPerros, AJ
dc.contributor.authorEsguerra-Lallen, A
dc.contributor.authorRooks, K
dc.contributor.authorChong, F
dc.contributor.authorEngkilde-Pedersen, S
dc.contributor.authorFaddy, HM
dc.contributor.authorHewlett, E
dc.contributor.authorNaidoo, R
dc.contributor.authorTung, JP
dc.contributor.authorFraser, JF
dc.contributor.authorTesar, P
dc.contributor.authorZiegenfuss, M
dc.contributor.authorSmith, S
dc.contributor.authorO’Brien, D
dc.contributor.authorFlower, RL
dc.contributor.authorDean, MM
dc.date.accessioned2020-05-11T23:50:06Z
dc.date.available2020-05-11T23:50:06Z
dc.date.issued2020
dc.identifier.issn1582-1838
dc.identifier.doi10.1111/jcmm.15154
dc.identifier.urihttp://hdl.handle.net/10072/393785
dc.description.abstractCoronary artery bypass grafting (CABG) triggers a systemic inflammatory response that may contribute to adverse outcomes. Dendritic cells (DC) and monocytes are immunoregulatory cells potentially affected by CABG, contributing to an altered immune state. This study investigated changes in DC and monocyte responses in CABG patients at 5 time-points: admission, peri-operative, ICU, day 3 and day 5. Whole blood from 49 CABG patients was used in an ex vivo whole blood culture model to prospectively assess DC and monocyte responses. Lipopolysaccharide (LPS) was added in parallel to model responses to an infectious complication. Co-stimulatory and adhesion molecule expression and intracellular mediator production was measured by flow cytometry. CABG modulated monocyte and DC responses. In addition, DC and monocytes were immunoparalysed, evidenced by failure of co-stimulatory and adhesion molecules (eg HLA-DR), and intracellular mediators (eg IL-6) to respond to LPS stimulation. DC and monocyte modulation was associated with prolonged ICU length of stay and post-operative atrial fibrillation. DC and monocyte cytokine production did not recover by day 5 post-surgery. This study provides evidence that CABG modulates DC and monocyte responses. Using an ex vivo model to assess immune competency of CABG patients may help identify biomarkers to predict adverse outcomes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofpagefrom4791
dc.relation.ispartofpageto4803
dc.relation.ispartofissue8
dc.relation.ispartofjournalJournal of Cellular and Molecular Medicine
dc.relation.ispartofvolume24
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1103
dc.subject.keywordsDendritic cells
dc.subject.keywordscardiac surgery
dc.subject.keywordscoronary artery bypass
dc.subject.keywordsimmune modulation
dc.subject.keywordsimmunoparalysis
dc.titleCoronary artery bypass grafting is associated with immunoparalysis of monocytes and dendritic cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationPerros, AJ; Esguerra-Lallen, A; Rooks, K; Chong, F; Engkilde-Pedersen, S; Faddy, HM; Hewlett, E; Naidoo, R; Tung, JP; Fraser, JF; Tesar, P; Ziegenfuss, M; Smith, S; O’Brien, D; Flower, RL; Dean, MM, Coronary artery bypass grafting is associated with immunoparalysis of monocytes and dendritic cells, Journal of Cellular and Molecular Medicine, 2020, 24 (8), pp. 4791-4803
dcterms.dateAccepted2020-02-10
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-05-11T23:45:44Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 Australian Red Cross Lifeblood. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorFraser, John F.


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