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dc.contributor.authorGolbert, Daiane Cristina F
dc.contributor.authorSantana-Van-Vliet, Eliane
dc.contributor.authorRibeiro-Alves, Marcelo
dc.contributor.authorda Fonseca, Marbella Maria B
dc.contributor.authorLepletier, Ailin
dc.contributor.authorMendes-da-Cruz, Daniella Areas
dc.contributor.authorLoss, Guilherme
dc.contributor.authorCotta-de-Almeida, Vinicius
dc.contributor.authorVasconcelos, Ana Tereza R
dc.contributor.authorSavino, Wilson
dc.date.accessioned2020-05-18T05:00:34Z
dc.date.available2020-05-18T05:00:34Z
dc.date.issued2018
dc.identifier.issn1933-6918
dc.identifier.doi10.1080/19336918.2017.1327513
dc.identifier.urihttp://hdl.handle.net/10072/393948
dc.description.abstractThe thymus supports differentiation of T cell precursors. This process requires relocation of developing thymocytes throughout multiple microenvironments of the organ, mainly with thymic epithelial cells (TEC), which control intrathymic T cell differentiation influencing the formation and maintenance of the immunological synapse. In addition to the proteins of the major histocompatibility complex (MHC), this structure is supported by several adhesion molecules. During the process of thymopoiesis, we previously showed that laminin-mediated interactions are involved in the entrance of T-cell precursors into the thymus, as well as migration of differentiating thymocytes within the organ. Using small interference RNA strategy, we knocked-down the ITGA6 gene (which encodes the CD49f integrin α-chain) in cultured human TEC, generating a decrease in the expression of the corresponding CD49f subunit, in addition to modulation in several other genes related to cell adhesion and migration. Thymocyte adhesion to TEC was significantly impaired, comprising both immature and mature thymocyte subsets. Moreover, we found a modulation of the MHC, with a decrease in membrane expression of HLA-ABC, in contrast with increase in the expression of HLA-DR. Interestingly, the knockdown of the B2M gene (encoding the β-2 microglobulin of the HLA-ABC complex) increased CD49f expression levels, thus unraveling the existence of a cross-talk event in the reciprocal control of CD49f and HLA-ABC. Our data suggest that the expression levels of CD49f may be relevant in the general control of MHC expression by TEC and consequently the corresponding synapse with developing thymocytes mediated by the T-cell receptor.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherTaylor & Francis
dc.relation.ispartofpagefrom152
dc.relation.ispartofpageto167
dc.relation.ispartofissue2
dc.relation.ispartofjournalCell Adhesion & Migration
dc.relation.ispartofvolume12
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode0699
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCell Biology
dc.subject.keywordsextracellular matrix
dc.subject.keywordsHLA-ABC
dc.titleSmall interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGolbert, DCF; Santana-Van-Vliet, E; Ribeiro-Alves, M; da Fonseca, MMB; Lepletier, A; Mendes-da-Cruz, DA; Loss, G; Cotta-de-Almeida, V; Vasconcelos, ATR; Savino, W, Small interference ITGA6 gene targeting in the human thymic epithelium differentially regulates the expression of immunological synapse-related genes, Cell Adhesion & Migration, 2018, 12 (2), pp. 152-167
dcterms.licensehttp://creativecommons.org/licenses/by-nc/3.0/
dc.date.updated2020-05-18T04:25:55Z
dc.description.versionPublished
gro.rights.copyright© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLepletier de Oliveira, Ailin


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