Intra-population strain variation in phosphorus storage strategies of the freshwater cyanobacterium Raphidiopsis raciborskii
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Author(s)
Xiao, M
Hamilton, DP
Chuang, A
Burford, MA
Year published
2020
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Several cyanobacteria, including diazotrophic Raphidiopsis raciborskii, can form harmful blooms when dissolved inorganic phosphorus (DIP) concentrations are very low. We hypothesized that R. raciborskii strains would vary in P allocations to cell growth and storage, providing resilience of populations to continuously low or variable P supplies. We tested this hypothesis using six toxic strains (producing cylindrospermopsins) isolated from a field population using batch monocultures with and without P and dissolved inorganic nitrogen (DIN). Treatments replete with DIN, irrespective of P addition, had similar exponential growth ...
View more >Several cyanobacteria, including diazotrophic Raphidiopsis raciborskii, can form harmful blooms when dissolved inorganic phosphorus (DIP) concentrations are very low. We hypothesized that R. raciborskii strains would vary in P allocations to cell growth and storage, providing resilience of populations to continuously low or variable P supplies. We tested this hypothesis using six toxic strains (producing cylindrospermopsins) isolated from a field population using batch monocultures with and without P and dissolved inorganic nitrogen (DIN). Treatments replete with DIN, irrespective of P addition, had similar exponential growth rates for individual strains. P storage capacity varied four-fold among strains and was significantly higher in DIN free treatments than in replete treatments. P was stored by all R. raciborskii strains, in preference to allocation to increase growth rates. P stores decreased with increased growth rate across strains but were not related to the time to P starvation in P free treatments. The storage capacity of R. raciborskii, combined with strategies to efficiently uptake P, mean that P controls may not control R. raciborskii populations in the short-term. Intra-population strain variation in P storage capacity will need to be reflected in process-based models to predict blooms of R. raciborskii and other cyanobacteria adapted to low-P conditions.
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View more >Several cyanobacteria, including diazotrophic Raphidiopsis raciborskii, can form harmful blooms when dissolved inorganic phosphorus (DIP) concentrations are very low. We hypothesized that R. raciborskii strains would vary in P allocations to cell growth and storage, providing resilience of populations to continuously low or variable P supplies. We tested this hypothesis using six toxic strains (producing cylindrospermopsins) isolated from a field population using batch monocultures with and without P and dissolved inorganic nitrogen (DIN). Treatments replete with DIN, irrespective of P addition, had similar exponential growth rates for individual strains. P storage capacity varied four-fold among strains and was significantly higher in DIN free treatments than in replete treatments. P was stored by all R. raciborskii strains, in preference to allocation to increase growth rates. P stores decreased with increased growth rate across strains but were not related to the time to P starvation in P free treatments. The storage capacity of R. raciborskii, combined with strategies to efficiently uptake P, mean that P controls may not control R. raciborskii populations in the short-term. Intra-population strain variation in P storage capacity will need to be reflected in process-based models to predict blooms of R. raciborskii and other cyanobacteria adapted to low-P conditions.
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Journal Title
FEMS Microbiology Ecology
Copyright Statement
© 2020 Oxford University Press. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in FEMS Microbiology Ecology following peer review. The definitive publisher-authenticated version Intra-population strain variation in phosphorus storage strategies of the freshwater cyanobacterium Raphidiopsis raciborskii, FEMS Microbiology Ecology, 2020 is available online at: DOI.
Subject
Freshwater ecology
Plant biochemistry
Environmental management
Microbiology
Raphidiopsis raciborskii (Cylindrospermopsis raciborskii)
Carbon
cylindrospermopsins (CYNs)
nitrogen
photosynthetic yield