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dc.contributor.authorShapiro, Amanda
dc.contributor.authorBosward, Katrina
dc.contributor.authorMathews, Karen
dc.contributor.authorVincent, Gemma
dc.contributor.authorStenos, John
dc.contributor.authorTadepalli, Mythili
dc.contributor.authorNorris, Jacqueline
dc.date.accessioned2020-06-01T02:58:05Z
dc.date.available2020-06-01T02:58:05Z
dc.date.issued2020
dc.identifier.issn1863-1959
dc.identifier.doi10.1111/zph.12707
dc.identifier.urihttp://hdl.handle.net/10072/394259
dc.description.abstractThe discovery of antibodies against Coxiella burnetii in cattery‐confined breeding cats indicating prior or current exposure (Shapiro et al. , 2015) prompted an investigation into possible sources of infection. One hypothesis was that raw meat diets containing reservoir species may provide a source of C. burnetii transmission. The aim of this pilot study was to determine whether C. burnetii DNA was present in raw meat sold exclusively for companion animal consumption. The sample population consisted of raw meat packages (n = 58) of primarily kangaroo origin, with three to four aliquots (50–120 mg) randomly selected from each package. Genomic DNA was extracted from whole tissue in each of these aliquots using a modified protocol. Three quantitative PCR assays were used for the detection of C. burnetii targeting the IS1111 gene, the heat shock operon htpAB and the C. burnetii outer membrane protein‐coding gene, com1 . Coxiella burnetii DNA was detected in 25/58 samples (43%) using the IS1111 , htpAB and/or com1 PCR assays and confirmed by DNA sequencing. All samples amplifying a product in the com1 assay also amplified a product in the htpAB and IS1111 assays. A total of 17/58 (29%) packets were positive with all three genes, 4/58 (7%) were positive with two genes (IS1111 and htpAB ) and 4/58 (7%) were positive with the IS1111 gene only. Coxiella burnetii DNA was five times more likely to be found in offal than skeletal muscle meat samples. All meat samples in which C. burnetii DNA was found were from kangaroo tissues, while samples labelled as non‐kangaroo meat (n = 4) were negative. Multi‐locus variable number of tandem repeat analysis (MLVA) identified three different genotypes of C. burnetii that have all been identified previously from Australian human clinical Q fever cases. Further investigations are required to determine the potential role of certain raw meats in the transmission of C. burnetii to cats and humans.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofpagefrom443
dc.relation.ispartofpageto452
dc.relation.ispartofissue4
dc.relation.ispartofjournalZoonoses and Public Health
dc.relation.ispartofvolume67
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsPublic, Environmental & Occupational Health
dc.subject.keywordsInfectious Diseases
dc.subject.keywordsVeterinary Sciences
dc.titleMolecular detection of Coxiella burnetii in raw meat intended for pet consumption
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationShapiro, A; Bosward, K; Mathews, K; Vincent, G; Stenos, J; Tadepalli, M; Norris, J, Molecular detection of Coxiella burnetii in raw meat intended for pet consumption, Zoonoses and Public Health, 2020, 67 (4), Pages 443-452
dcterms.dateAccepted2020-03-20
dc.date.updated2020-06-01T01:14:37Z
gro.hasfulltextNo Full Text
gro.griffith.authorStenos, John


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