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dc.contributor.authorCai, Pengfei
dc.contributor.authorMu, Yi
dc.contributor.authorOlveda, Remigio M
dc.contributor.authorRoss, Allen G
dc.contributor.authorOlveda, David U
dc.contributor.authorMcManus, Donald P
dc.date.accessioned2020-06-02T23:45:02Z
dc.date.available2020-06-02T23:45:02Z
dc.date.issued2020
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms21103560
dc.identifier.urihttp://hdl.handle.net/10072/394327
dc.description.abstractChronic infection with Schistosoma japonicum or Schistosoma mansoni results in hepatic fibrosis of the human host. The staging of fibrosis is crucial for prognosis and to determine the need for treatment of patients with schistosomiasis. This study aimed to determine whether there is a correlation between the levels of serum exosomal micro-ribonucleic acids (miRNAs) (exomiRs) and fibrosis progression in schistosomiasis. Reference gene (RG) validation was initially carried out for the analysis of serum exomiRs expression in staging liver fibrosis caused by schistosome infection. The expression levels of liver fibrosis-associated exomiRs in serum were determined in a murine schistosomiasis model and in a cohort of Filipino schistosomiasis japonica patients (n = 104) with different liver fibrosis grades. Of twelve RG candidates validated, miR-103a-3p and miR-425-5p were determined to be the most stable genes in the murine schistosomiasis model and subjects from the schistosomiasis-endemic area, respectively. The temporal expression profiles of nine fibrosis-associated serum exomiRs, as well as their correlations with the liver pathologies, were determined in C57BL/6 mice during S. japonicum infection. The serum levels of three exomiRs (miR-92a-3p, miR-146a-5p and miR-532-5p) were able to distinguish subjects with fibrosis grades I-III from those with no fibrosis, but only the serum level of exosomal miR-146a-5p showed potential for distinguishing patients with mild (grades 0-I) versus severe fibrosis (grades II-III). The current data imply that serum exomiRs can be a supplementary tool for grading liver fibrosis in hepatosplenic schistosomiasis with moderate accuracy.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom3560:1
dc.relation.ispartofpageto3560:18
dc.relation.ispartofissue10
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume21
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1037304
dc.relation.grantIDAPP1037304
dc.relation.fundersNHMRC
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther biological sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3199
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3404
dc.subject.keywordsSchistosoma japonicum
dc.subject.keywordsbiomarker
dc.subject.keywordsexomiRs
dc.subject.keywordsexosomal microRNAs
dc.subject.keywordshepatic fibrosis
dc.titleSerum Exosomal miRNAs for Grading Hepatic Fibrosis Due to Schistosomiasis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationCai, P; Mu, Y; Olveda, RM; Ross, AG; Olveda, DU; McManus, DP, Serum Exosomal miRNAs for Grading Hepatic Fibrosis Due to Schistosomiasis, International Journal of Molecular Sciences, 2020, 21 (10), pp. 3560:1-3560:18
dcterms.dateAccepted2020-05-14
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-06-02T05:04:41Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorRoss, Allen G.


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