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dc.contributor.authorSpeicher, David J
dc.contributor.authorLuinstra, Kathy
dc.contributor.authorSmith, Emma J
dc.contributor.authorCastriciano, Santina
dc.contributor.authorSmieja, Marek
dc.date.accessioned2020-06-07T23:25:44Z
dc.date.available2020-06-07T23:25:44Z
dc.date.issued2020
dc.identifier.issn0732-8893
dc.identifier.doi10.1016/j.diagmicrobio.2020.115038
dc.identifier.urihttp://hdl.handle.net/10072/394454
dc.description.abstractSalivary antibodies are useful in surveillance and vaccination studies. However, low antibody levels and degradation by endonucleases are problematic. Oral flocked swabs are a potential non-invasive alternative for detecting viral antibodies. Seroprevalence for Cytomegalovirus (CMV), Varicella-Zoster virus (VZV), Epstein–Barr virus (EBV), Measles and Mumps IgG antibodies were determined from 50 matched serum, saliva and swabs samples from healthy volunteers using commercial ELISAs. CMV IgG, VZV IgG, and EBV EBNA-1 IgG, VCA IgG, and Measles IgG swab versus serum sensitivities were 95.8%, 96.0%, 92.1%, 95.5%, 84.5%, respectively, and swabs correlated well with saliva. Sensitivity of Mumps IgG in swabs and saliva was poor at 60.5%, and 68.2%, respectively. Specificities for IgG antibodies were 100% for CMV, EBV and Mumps, but could not be determined for VZV and Measles due to exclusively seropositive volunteers. Except for Mumps IgG, swabs correlate well with serum, are easy to self-collect and are stable at room temperature.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom115038:1
dc.relation.ispartofpageto115038:7
dc.relation.ispartofjournalDiagnostic Microbiology and Infectious Disease
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1108
dc.subject.keywordsCytomegalovirus
dc.subject.keywordsDiagnostics
dc.subject.keywordsEpstein–Barr virus
dc.subject.keywordsMeasles
dc.subject.keywordsMumps
dc.titleNon-invasive detection of IgG antibodies from common pathogenic viruses using oral flocked swabs
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSpeicher, DJ; Luinstra, K; Smith, EJ; Castriciano, S; Smieja, M, Non-invasive detection of IgG antibodies from common pathogenic viruses using oral flocked swabs, Diagnostic Microbiology and Infectious Disease, 2020, pp. 115038:1-115038:7
dcterms.dateAccepted2020-03-10
dc.date.updated2020-06-07T22:38:49Z
gro.hasfulltextNo Full Text
gro.griffith.authorSpeicher, David J.


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