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dc.contributor.authorLoisy, F.en_US
dc.contributor.authorAtmar, R.en_US
dc.contributor.authorGuillon, P.en_US
dc.contributor.authorLe Cann, P.en_US
dc.contributor.authorPommepuy, M.en_US
dc.contributor.authorLe Guyader, F.en_US
dc.date.accessioned2017-04-24T13:09:57Z
dc.date.available2017-04-24T13:09:57Z
dc.date.issued2005en_US
dc.date.modified2011-07-09T02:31:08Z
dc.identifier.issn01660934en_US
dc.identifier.doi10.1016/j.jviromet.2004.08.023en_AU
dc.identifier.urihttp://hdl.handle.net/10072/39466
dc.description.abstractReal-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II. Seventy and seven RT-PCR units of genogroup I and II reference norovirus strains, respectively, were detected in artificially contaminated oysters. Validation of the new method on 150 archived naturally contaminated shellfish confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, but genogroup I strains were detected infrequently.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom1en_US
dc.relation.ispartofpageto7en_US
dc.relation.ispartofissue1en_US
dc.relation.ispartofjournalJournal of Virological Methodsen_US
dc.relation.ispartofvolume123en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchVirologyen_US
dc.subject.fieldofresearchcode060506en_US
dc.titleReal-time RT-PCR for norovirus screening in shellfishen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2005
gro.hasfulltextNo Full Text


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