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dc.contributor.authorMatin, Farhana
dc.contributor.authorJeet, Varinder
dc.contributor.authorSrinivasan, Srilakshmi
dc.contributor.authorCristino, Alexandre S
dc.contributor.authorPanchadsaram, Janaththani
dc.contributor.authorClements, Judith A
dc.contributor.authorBatra, Jyotsna
dc.contributor.authorAustralian Prostate Cancer BioResource
dc.description.abstractBACKGROUND: MicroRNAs mediate biological processes through preferential binding to the 3′ untranslated region (3′ UTR) of target genes. Studies have shown their association with prostate cancer (PCa) risk through single-nucleotide polymorphisms (SNPs), known as miRSNPs. In a European cohort, 22 PCa risk-associated miRSNPs have been identified. The most significant miRSNP in the 3′ UTR of Kallikrein-related peptidase 3 (KLK3) created a binding site for miR-3162-5p. Here we investigated the miR-3162-5p–KLK interaction and the clinical implication of miR-3162-5p in PCa. METHODS: We tested the role of miR-3162-5p in PCa etiology using IncuCyte live-cell imaging and anchorage-independent growth assays. The effect of miR-3162-5p on KLK and androgen receptor (AR) expression was measured by RT-quantitative (q)PCR and target pulldown assays. KLK3 proteolytic activity was determined by DELFIA® immunoassay. Mass spectrometry identified pathways affected by miR-3162-5p. miR-3162-5p expression was measured in clinical samples using RT-qPCR. RESULTS: miR-3162-5p affected proliferation, migration, and colony formation of LNCaP cells by regulating the expression of KLK2–4 and AR by direct targeting. KLK3 protein expression was regulated by miR-3162-5p consistent with lower KLK3 proteolytic activity observed in LNCaP-conditioned media. KLK/AR pulldown and mass spectrometry analysis showed a potential role of miR-3162-5p in metabolic pathways via KLK/AR and additional targets. Increased miR-3162-5p expression was observed in prostate tumor tissues with higher Gleason grade. CONCLUSIONS: Our study provides an insight into possible involvement of miR-3162-5p in PCa etiology by targeting KLKs and AR. It highlights clinical utility of miR-3162-5p and its interactive axis as a new class of biomarkers and therapeutic targets for PCa.
dc.publisherOxford University Press (OUP)
dc.relation.ispartofjournalClinical Chemistry
dc.subject.fieldofresearchMedical biotechnology
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchClinical sciences
dc.titleMicroRNA-3162-5p-Mediated Crosstalk between Kallikrein Family Members Including Prostate-Specific Antigen in Prostate Cancer
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMatin, F; Jeet, V; Srinivasan, S; Cristino, AS; Panchadsaram, J; Clements, JA; Batra, J, MicroRNA-3162-5p-Mediated Crosstalk between Kallikrein Family Members Including Prostate-Specific Antigen in Prostate Cancer, Clinical Chemistry, 2019, 65 (6), pp. 771-780
gro.hasfulltextNo Full Text
gro.griffith.authorCristino, Alex

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