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dc.contributor.authorHo, Chee Meng Benjamin
dc.contributor.authorSun, Qi
dc.contributor.authorTeo, Adrian JT
dc.contributor.authorWibowo, David
dc.contributor.authorGao, Yongsheng
dc.contributor.authorZhou, Jun
dc.contributor.authorHuang, Yanyi
dc.contributor.authorTan, Say Hwa
dc.contributor.authorZhao, Chun-Xia
dc.date.accessioned2020-07-09T00:20:59Z
dc.date.available2020-07-09T00:20:59Z
dc.date.issued2020
dc.identifier.issn2373-9878
dc.identifier.doi10.1021/acsbiomaterials.0c00292
dc.identifier.urihttp://hdl.handle.net/10072/395250
dc.description.abstractDroplet microfluidics creates new opportunities for microbial engineering. Most microbial cultivations are carried out in bioreactors, which are usually bulky and consume a large amount of reagents and media. In this paper, we propose a microfluidic droplet-based microbioreactor for microbial cultivation. A microfluidic device was designed and fabricated to produce many droplet-based microbioreactors integrated with an AC electric field for the manipulation of these microbioreactors. Droplets encapsulating fluorescent Escherichia coli cells were generated, sorted, and trapped individually in small chambers. Fluorescence intensity was monitored to determine cell growth. An electric field with varying voltages and frequencies manipulates the droplets, simulating an oscillation effect. Initial results showed that electric field does not affect cell growth. A comparison with shake flask showed that a similar standard growth curve is obtained when cultivating at room temperature. This device has the potential for making droplet-based microbioreactors an alternative for microbial engineering research.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofpagefrom3630
dc.relation.ispartofpageto3637
dc.relation.ispartofissue6
dc.relation.ispartofjournalACS Biomaterials Science & Engineering
dc.relation.ispartofvolume6
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchcode09
dc.subject.fieldofresearchcode0903
dc.titleDevelopment of a Microfluidic Droplet-Based Microbioreactor for Microbial Cultivation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHo, CMB; Sun, Q; Teo, AJT; Wibowo, D; Gao, Y; Zhou, J; Huang, Y; Tan, SH; Zhao, C-X, Development of a Microfluidic Droplet-Based Microbioreactor for Microbial Cultivation, ACS Biomaterials Science & Engineering, 2020, 6 (6), pp. 3630-3637
dc.date.updated2020-07-08T23:12:34Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyrightThis document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science and Engineering, © 2020 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/acsbiomaterials.0c00292
gro.hasfulltextFull Text
gro.griffith.authorWibowo, David
gro.griffith.authorHo, Benjamin
gro.griffith.authorTeo, Adrian J.
gro.griffith.authorGao, Yongsheng
gro.griffith.authorZhou, Jun
gro.griffith.authorTan, Say Hwa H.


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