Show simple item record

dc.contributor.authorLiu, Jianping
dc.contributor.authorDuan, Chengchen
dc.contributor.authorZhang, Wenzhu
dc.contributor.authorTa, Hang Thu
dc.contributor.authorYuan, Jingli
dc.contributor.authorZhang, Run
dc.contributor.authorXu, Zhi Ping
dc.date.accessioned2020-07-10T01:50:37Z
dc.date.available2020-07-10T01:50:37Z
dc.date.issued2020
dc.identifier.issn0003-2670
dc.identifier.doi10.1016/j.aca.2019.12.056
dc.identifier.urihttp://hdl.handle.net/10072/395305
dc.description.abstractGasotransmitter hydrogen sulfide (H2S), produced enzymatically in body, has important functions in biological signaling and metabolic processes. An abnormal level of H2S expression is associated with different diseases, therefore, development of novel bioanalytical methods for rapid and effective detection of H2S in biological conditions is of great importance. In this work, we report the development of a new responsive nanosensor for ratiometric luminescence detection of H2S in aqueous solution and live cells. The nanosensor (Ru@FITC-MSN) was prepared by immobilizing a luminescent ruthenium(II) (Ru(II)) complex into a fluorescein isothiocyanate (FITC) conjugated water-dispersible mesoporous silica nanoparticle (MSN), showing dual emission bands at 520 nm (FITC) and 600 nm (Ru complex). The red luminescence of the formed Ru@FITC-MSN was quenched in the presence of Cu2+. The in-situ generated Ru–Cu@FITC-MSN responded to H2S rapidly and selectively, showing a linear ratiometric luminescence change in FITC and Ru(II) channels with the H2S concentration (0.5–4 μM). Limit of detection (LoD) and limit of quantification (LoQ) were determined to be 0.36 and 1.21 μM. Followed by investigation of cellular uptake processes, the utility of the nanosensor for ratiometric imaging of H2S in live cells and its capability to monitor H2S levels in inflammatory breast cancer cells were then demonstrated. This study provides a powerful approach for detection of highly reactive and unstable H2S biomolecules in live systems.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom156
dc.relation.ispartofpageto163
dc.relation.ispartofjournalAnalytica Chimica Acta
dc.relation.ispartofvolume1103
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3499
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsChemistry
dc.subject.keywordsResponsive nanosensor
dc.titleResponsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLiu, J; Duan, C; Zhang, W; Ta, HT; Yuan, J; Zhang, R; Xu, ZP, Responsive nanosensor for ratiometric luminescence detection of hydrogen sulfide in inflammatory cancer cells, Analytica Chimica Acta, 2020, 1103, pp. 156-163
dcterms.dateAccepted2019-12-19
dc.date.updated2020-07-10T01:45:47Z
gro.hasfulltextNo Full Text
gro.griffith.authorTa, Hang


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record