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dc.contributor.authorSreejith, Kamalalayam Rajan
dc.contributor.authorGorgannezhad, Lena
dc.contributor.authorJin, Jing
dc.contributor.authorOoi, Chin Hong
dc.contributor.authorTakei, Takayuki
dc.contributor.authorHayase, Gen
dc.contributor.authorStratton, Helen
dc.contributor.authorLamb, Krystina
dc.contributor.authorShiddiky, Muhammad
dc.contributor.authorDao, Dzung Viet
dc.contributor.authorNguyen, Nam-Trung
dc.date.accessioned2020-07-13T00:43:20Z
dc.date.available2020-07-13T00:43:20Z
dc.date.issued2020
dc.identifier.issn2072-666X
dc.identifier.doi10.3390/mi11030242
dc.identifier.urihttp://hdl.handle.net/10072/395364
dc.description.abstractOver the last three decades, the protocols and procedures of the DNA amplification technique, polymerase chain reaction (PCR), have been optimized and well developed. However, there have been no significant innovations in processes for sample dispersion for PCR that have reduced the amount of single-use or unrecyclable plastic waste produced. To address the issue of plastic waste, this paper reports the synthesis and successful use of a core-shell bead microreactor using photopolymerization of a composite liquid marble as a dispersion process. This platform uses the core-shell bead as a simple and effective sample dispersion medium that significantly reduces plastic waste generated compared to conventional PCR processes. Other improvements over conventional PCR processes of the novel dispersion platform include increasing the throughput capability, enhancing the performance and portability of the thermal cycler, and allowing for the contamination-free storage of samples after thermal cycling.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherMDPI
dc.relation.ispartofpagefrom242:1
dc.relation.ispartofpageto242:12
dc.relation.ispartofissue3
dc.relation.ispartofjournalMicromachines
dc.relation.ispartofvolume11
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode1007
dc.subject.keywordsScience & Technology
dc.subject.keywordsInstruments & Instrumentation
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsNanoscience
dc.titleCore-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSreejith, KR; Gorgannezhad, L; Jin, J; Ooi, CH; Takei, T; Hayase, G; Stratton, H; Lamb, K; Shiddiky, M; Dao, DV; Nguyen, N-T, Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction, Micromachines, 2020, 11 (3), pp. 242:1-242:12
dcterms.dateAccepted2020-02-25
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-07-13T00:35:38Z
dc.description.versionPublished
gro.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLamb, Krystina
gro.griffith.authorDao, Dzung V.
gro.griffith.authorGorgannezhad, Lena
gro.griffith.authorJin, Jing
gro.griffith.authorKamalalayam Rajan, Sreejith
gro.griffith.authorOoi, Chin Hong
gro.griffith.authorStratton, Helen M.
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorNguyen, Nam-Trung


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