dc.contributor.author | Sreejith, Kamalalayam Rajan | |
dc.contributor.author | Gorgannezhad, Lena | |
dc.contributor.author | Jin, Jing | |
dc.contributor.author | Ooi, Chin Hong | |
dc.contributor.author | Takei, Takayuki | |
dc.contributor.author | Hayase, Gen | |
dc.contributor.author | Stratton, Helen | |
dc.contributor.author | Lamb, Krystina | |
dc.contributor.author | Shiddiky, Muhammad | |
dc.contributor.author | Dao, Dzung Viet | |
dc.contributor.author | Nguyen, Nam-Trung | |
dc.date.accessioned | 2020-07-13T00:43:20Z | |
dc.date.available | 2020-07-13T00:43:20Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2072-666X | |
dc.identifier.doi | 10.3390/mi11030242 | |
dc.identifier.uri | http://hdl.handle.net/10072/395364 | |
dc.description.abstract | Over the last three decades, the protocols and procedures of the DNA amplification technique, polymerase chain reaction (PCR), have been optimized and well developed. However, there have been no significant innovations in processes for sample dispersion for PCR that have reduced the amount of single-use or unrecyclable plastic waste produced. To address the issue of plastic waste, this paper reports the synthesis and successful use of a core-shell bead microreactor using photopolymerization of a composite liquid marble as a dispersion process. This platform uses the core-shell bead as a simple and effective sample dispersion medium that significantly reduces plastic waste generated compared to conventional PCR processes. Other improvements over conventional PCR processes of the novel dispersion platform include increasing the throughput capability, enhancing the performance and portability of the thermal cycler, and allowing for the contamination-free storage of samples after thermal cycling. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | MDPI | |
dc.relation.ispartofpagefrom | 242:1 | |
dc.relation.ispartofpageto | 242:12 | |
dc.relation.ispartofissue | 3 | |
dc.relation.ispartofjournal | Micromachines | |
dc.relation.ispartofvolume | 11 | |
dc.subject.fieldofresearch | Nanotechnology | |
dc.subject.fieldofresearchcode | 4018 | |
dc.subject.keywords | Science & Technology | |
dc.subject.keywords | Instruments & Instrumentation | |
dc.subject.keywords | Science & Technology - Other Topics | |
dc.subject.keywords | Nanoscience | |
dc.title | Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Sreejith, KR; Gorgannezhad, L; Jin, J; Ooi, CH; Takei, T; Hayase, G; Stratton, H; Lamb, K; Shiddiky, M; Dao, DV; Nguyen, N-T, Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction, Micromachines, 2020, 11 (3), pp. 242:1-242:12 | |
dcterms.dateAccepted | 2020-02-25 | |
dcterms.license | https://creativecommons.org/licenses/by/4.0/ | |
dc.date.updated | 2020-07-13T00:35:38Z | |
dc.description.version | Version of Record (VoR) | |
gro.rights.copyright | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Lamb, Krystina | |
gro.griffith.author | Dao, Dzung V. | |
gro.griffith.author | Kamalalayam Rajan, Sreejith | |
gro.griffith.author | Ooi, Chin Hong | |
gro.griffith.author | Stratton, Helen M. | |
gro.griffith.author | Shiddiky, Muhammad J. | |
gro.griffith.author | Nguyen, Nam-Trung | |