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dc.contributor.authorLiu, Menghe
dc.contributor.authorHummitzsch, Katja
dc.contributor.authorHartanti, Monica D
dc.contributor.authorRosario, Roseanne
dc.contributor.authorBastian, Nicole A
dc.contributor.authorHatzirodos, Nicholas
dc.contributor.authorBonner, Wendy M
dc.contributor.authorIrving-Rodgers, Helen F
dc.contributor.authorLaven, Joop SE
dc.contributor.authorAnderson, Richard A
dc.contributor.authorRodgers, Raymond J
dc.date.accessioned2020-07-30T04:47:40Z
dc.date.available2020-07-30T04:47:40Z
dc.date.issued2020
dc.identifier.issn1529-7268
dc.identifier.doi10.1093/biolre/ioaa119
dc.identifier.urihttp://hdl.handle.net/10072/395938
dc.description.abstractPolycystic ovary syndrome (PCOS) appears to have a genetic predisposition and a fetal origin. We compared the expression levels of 25 PCOS candidate genes from adult control and PCOS human ovaries (n = 16) using microarrays. Only one gene was potentially statistically different. Using qRT-PCR, expression of PCOS candidate genes was examined in bovine fetal ovaries from early stages when they first developed stroma through to completion of development (n = 27; 60-270 days of gestation). The levels of ERBB3 mRNA negatively correlated with gestational age but positively with HMGA2, FBN3, TOX3, GATA4 and DENND1A.X1,2,3,4, previously identified as correlated with each other and expressed early. PLGRKT and ZBTB16, and less so IRF1, were also correlated with AMH, FSHR, AR, INSR and TGFB1I1, previously identified as correlated with each other and expressed late. ARL14EP, FDFT1, NEIL2 and MAPRE1 were expressed across gestation and not correlated with gestational age as shown previously for THADA, ERBB4, RAD50, C8H9orf3, YAP1, RAB5B, SUOX and KRR1. LGCGR because of its unusual bimodal expression pattern had some unusual correlations with other genes. In human ovaries (n = 15, < 150 days of gestation), ERBB3.V1 and ERBB3.VS were expressed and correlated negatively with gestational age and positively with FBN3, HMGA2, DENND1A.V1,3,4, DENND1A.V1-7, GATA4 and FSHR, previously identified as correlated with each other and expressed early. Thus, the general lack of differential expression of candidate genes in adult ovaries contrasting with dynamic patterns of gene expression in fetal ovaries is consistent with a vulnerability to disturbance in the fetal ovary that may underpin development of PCOS.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press (OUP)
dc.relation.ispartofjournalBiology of Reproduction
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode3202
dc.subject.keywordsadult ovary
dc.subject.keywordsfetal ovary
dc.subject.keywordsgene expression
dc.subject.keywordsgenetics
dc.subject.keywordspolycystic ovary syndrome
dc.titleAnalysis of expression of candidate genes for polycystic ovary syndrome in adult and fetal human and fetal bovine ovaries†
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLiu, M; Hummitzsch, K; Hartanti, MD; Rosario, R; Bastian, NA; Hatzirodos, N; Bonner, WM; Irving-Rodgers, HF; Laven, JSE; Anderson, RA; Rodgers, RJ, Analysis of expression of candidate genes for polycystic ovary syndrome in adult and fetal human and fetal bovine ovaries†., Biology of Reproduction, 2020
dc.date.updated2020-07-27T01:21:10Z
gro.hasfulltextNo Full Text
gro.griffith.authorIrving-Rodgers, Helen F.


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